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Debonding and hatching method for fertilized eggs of Thamnaconus septentrionalis

A technology of green finfish and fertilized eggs, which is applied in climate change adaptation, fish farming, application, etc., can solve the problems of perishability, aggravate the reproduction of germs, affect the hatching and seedling cultivation of fertilized eggs, and achieve the reduction of germs The effect of breeding and improving the water quality of growth

Inactive Publication Date: 2020-10-30
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, this method cannot separate fertilized eggs from unfertilized eggs well, and unfertilized eggs are easy to rot in the hatching tank, which will aggravate the reproduction of germs and affect the hatching of fertilized eggs and subsequent seedling cultivation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The method for debonding and hatching the fertilized eggs of the green-finned horse-faced fish comprises the following steps:

[0020] (1) Preparation of demucinating fluid and debonding of fertilized eggs: On May 23, 2018, at a water temperature of 19°C, the fertilized eggs were collected from the broodstock pond of the green finfish. Because the fertilized eggs were viscous and sinking, a large number of fertilized eggs sank. at the bottom of the pool. Lower the water level in the broodstock pond to 20cm, and collect the fertilized eggs at the bottom of the pond by dipping the net. Dissolve 25 g of alkaline protease (Bacillus licheniformis) (Solarbio) in about 5 L of seawater, mix well, and prepare a 5 g / L demucinating liquid.

[0021] Put 163g fertilized eggs (approximately 652,000 fertilized eggs) into the debonding solution, stir and mix continuously for 20 minutes, stop debonding when the fertilized eggs no longer stick to each other or adhere to the barrel wall,...

Embodiment 2

[0027] Use the conventional method to hatch the fertilized eggs of the green-finned horse-faced fish:

[0028] On May 24, 2018, when the water temperature was 19°C, the water level in the broodstock pond of the green finfish was lowered to 20 cm, and 152 g of fertilized eggs (about 608,000 fertilized eggs) were collected at the bottom of the pond through a plastic plate (attachment base). The fertilized eggs were continuously washed with clean sea water for 3 minutes, and impurities on the surface of the fertilized eggs were washed away as much as possible. Soak the fertilized eggs in 200mg / L povidone-iodine disinfectant for 5 minutes, and keep stirring to mix. Then wash the fertilized eggs with clean seawater for 3 minutes, and wash away the disinfectant. Sprinkle the sterilized fertilized eggs evenly into the hatching pool, the water temperature is 18-20°C, the salinity is 27-30, the dissolved oxygen is 6.0-8.0mg / L, and the pH is 7.8-8.2.

[0029] Using conventional method...

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PUM

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Abstract

The invention provides a debonding and hatching method for fertilized eggs of Thamnaconus septentrionalis, which belongs to the field of aquaculture and comprises the following steps: dissolving alkaline protease from bacillus licheniformis in seawater to prepare a 5g / L debonding solution; collecting fertilized eggs of the Thamnaconus septentrionalis, putting the fertilized eggs into a debonding solution, continuously stirring and uniformly mixing, stopping debonding when the fertilized eggs are not adhered to each other, are adhered to the barrel wall and are in a certain bright and transparent state, and separating the fertilized eggs from unfertilized eggs by increasing the salinity of seawater from the debonded fertilized eggs; washing the separated fertilized eggs clean and then hatching according to a normal procedure. According to the method, germ pollution in the hatching and seedling raising processes can be reduced, and the hatching rate and the emergence rate are increased.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to a method for debonding and hatching the fertilized eggs of the green-finned horse-faced fish. Background technique [0002] Green-finned horse-faced fish (Thamnaconus septentrionalis), commonly known as "skinned fish", "mouse fish" and "bread fish", is distributed in the Bohai Sea, the Yellow Sea and the East China Sea. It has delicious meat and rich nutrition. It is an excellent economic fish in my country's coastal areas. The breeding traits of green finfish are excellent, the survival temperature is 9-30°C, the omnivorous nature is easy to domesticate and breed, the feed utilization rate is high, and the growth rate is fast. It can be carried out in various modes such as industrial workshop culture, pond culture and cage culture. There are various product specifications, 100g can be sold, the early seedlings in the north can be cultivated and marketed in the same year, and the seas ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/17
CPCA01K61/17Y02A40/81
Inventor 边力陈四清李凤辉刘长琳葛建龙常青
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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