Egg cell multi-effect accurate electrical stimulation device and method thereof

Abstract

The invention discloses an egg cell multi-effect accurate electrical stimulation device. The multi-effect accurate electrical stimulation device comprises a base; an electrical stimulation mechanism comprising two first electrical stimulation assemblies and two second electrical stimulation assemblies, wherein each first electrical stimulation assembly comprises a liquid metal capillary microneedle electrode and a first needle holder connected with the liquid metal capillary microneedle electrode, and each second electrical stimulation assembly comprises a metal parallel plate microelectrode and a second needle holder connected with the metal parallel plate microelectrode; a cell pose adjusting chip arranged on the base and used for adjusting the pose of the cell; and a rotating mechanismarranged on the base, connected with the cell pose adjusting chip and used for driving the cell pose adjusting chip to rotate. The invention further discloses the egg cell multi-effect accurate electrical stimulation method. According to the invention, accurate electrical stimulation can be carried out on specific parts of egg cells in different stages in the whole cloning process, multiple effects are realized on targeted electrical stimulation in each stage of the somatic cell cloning process, and the success rate of the whole somatic cell cloning process is improved.

Description

technical field [0001] The invention relates to the field of biotechnology related to cloning, in particular to a multi-efficiency and precise electrical stimulation device and method for egg cells oriented to the cloning process. Background technique [0002] Cloning is the process of using biotechnology to produce offspring with the same genetic organization as the original individual through asexual reproduction. Since its birth, cloning technology has always occupied the most important position in life science research and related medical technology. Egg cells contain important biological genetic information and are the main operating objects of the cloning process. However, the success rate of cloning experiments is extremely low. One of the main reasons is that the viability of isolated egg cells will drop significantly, and they will easily die during the experiment. , At this time, it is necessary to artificially stimulate the egg cells to activate the cells. Amon...

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Problems solved by technology

However, due to the large distance between macroscopic parallel plate electrodes, the voltage required for applying electrical stimulation is also large, which is very easy to cause damage to the cells and is not safe; and the traditional electrical stimulator cannot complete the precise electrical stimulation of specific parts of the egg cells. It is also impossible to use a set of electrical stimulation devices to complete the various electrical stimulations required for cloning egg cells at various stages (electrical activation, electrical fusion, mitochondrial membrane potential increase, etc.)

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