A method for improving the effect of in vitro fertilization with thawed pig sperm without treating infertility
An in vitro fertilization, pig sperm technology, applied in biochemical equipment and methods, preservation of human or animal body, embryo cells, etc., can solve the problems of high polysperm fertilization rate, poor embryo quality, poor oocyte maturation quality , to achieve the effect of improving fertilization effect and enhancing activity
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Embodiment 1
[0025] A method for improving the effect of in vitro fertilization of pig sperm after thawing without treating infertility, comprising the following steps:
[0026] (1) The frozen semen is taken out and then thawed, wherein the frozen base fluid used for semen freezing is made of the following components by weight: 200 parts of glucose, 300 parts of lactose, 10 parts of sodium bicarbonate, 5 parts of penicillin sodium, 8 parts of streptomycin sulfate, 1800 parts of egg yolk, 7000 parts of three-distilled water, wherein phenethyl caffeate and polyvinylpyrrolidone are added to the frozen base liquid, and the molar concentration of caffeic acid phenethyl in the frozen base liquid is 30 μmol / L, the mass fraction of polyvinylpyrrolidone in the freezing base liquid is 0.4%;
[0027] (2) Wash the thawed semen with fertilization operation solution and centrifuge twice, then wash and centrifuge the centrifuged pellet once with mTBM capacitation solution, and add mTBM capacitation solu...
Embodiment 2
[0033] A method for improving the effect of in vitro fertilization of pig sperm after thawing without treating infertility, comprising the following steps:
[0034] (1) The frozen semen is taken out and then thawed, wherein the frozen base liquid used for semen freezing is made of the following components by weight: 250 parts of glucose, 350 parts of lactose, 13 parts of sodium bicarbonate, 6 parts of penicillin sodium, 10 parts of streptomycin sulfate, 2000 parts of egg yolk, 8000 parts of triple distilled water, wherein caffeic acid phenethyl and polyvinylpyrrolidone are added to the freezing base liquid, wherein the molar concentration of caffeic acid phenethyl in the freezing base liquid is 40 μmol / L, the mass fraction of polyvinylpyrrolidone in the freezing base liquid is 0.5%;
[0035] (2) Wash the thawed semen with the fertilization solution and centrifuge twice, then wash and centrifuge the centrifuged pellet once with mTBM capacitation solution, add mTBM capacitation...
Embodiment 3
[0041] A method for improving the effect of in vitro fertilization of pig sperm after thawing without treating infertility, comprising the following steps:
[0042] (1) The frozen semen is taken out and then thawed, wherein the frozen base liquid used for semen freezing is made of the following components by weight: 300 parts of glucose, 400 parts of lactose, 15 parts of sodium bicarbonate, 7 parts of penicillin sodium, 12 parts of streptomycin sulfate, 2200 parts of egg yolk, 9000 parts of triple distilled water, wherein caffeic acid phenethyl and polyvinylpyrrolidone are added to the freezing base liquid, wherein the molar concentration of caffeic acid phenethyl in the freezing base liquid is 50 μmol / L, the mass fraction of polyvinylpyrrolidone in the freezing base liquid is 0.6%;
[0043] (2) Wash the thawed semen with the fertilization solution and centrifuge twice, then wash and centrifuge the centrifuged pellet once with mTBM capacitation solution, add mTBM capacitation...
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