31results about How to "Improve freezing ability" patented technology

The invention discloses a streptococcus thermophilus freeze-drying protective agent and a preparation method thereof. The streptococcus thermophilus freeze-drying protective agent comprises the following components in parts by mass: 10-20 parts of dried skim milk, 3-10 parts of raffinose, 4-10 parts of soybean oligosaccharides, 1.05-5.0 parts of dextran D40, 0.3-0.8 parts of sodium tripolyphosphate, 0.005-0.02 parts of propylgallate and 100 parts of distilled water, wherein the soybean oligosaccharides and the dextran D40 can both be purchased from LUOSHENBO science and technology Ltd.. The anti-freezing ability of thallus can be increased by adding a freeze-drying protective agent during a thallus powder preparing process, thus the freeze-drying survival rate of the thallus powder can be increased, the storage period can be longer, and the survival rate can not be easily lowered.

The invention discloses a pipe casing type heat exchanger which comprises a casing, a heat exchange pipe arranged in the casing and sealing heads arranged at two ends of the heat exchanger. An inside-pipe fluid inlet and an inside-pipe fluid outlet are arranged on the sealing heads, and an outside-pipe fluid inlet and an outside-pipe fluid outlet are arranged on the casing. Two ends of the heat exchange pipe are connected with a pipe plate, the heat exchange pipe is a pipe bundle composed of at least one transformation pipe, and the transformation pipes are mutually contacted in a certain distance to form a self-support structure. The pipe casing type heat exchanger is capable of achieving the effect that fluid inside and outside the pipes flows in parallel. The heat exchanger has the advantages of being high in heat exchange efficiency, small in resistance, not apt to scaling, small in vibration and the like.

The invention discloses a method for improving the in vitro fertilization effect of thawed swine sperm without treating infertility, comprising the following operation steps: (1) taking out the frozen semen and then thawing it; (2) using the thawed semen with The fertilization operation solution was washed and centrifuged twice, and then the centrifuged sediment was washed and centrifuged once with mTBM capacitative solution, and then resuspended and cultured to obtain capacitative semen; (3) The ovary from which the follicular fluid was extracted was used to pick out the cumulus-egg under a microscope Mother cell complex, take out the oocyte after culturing, and remove the cumulus; (4) add capacitative semen to each fertilization droplet, put it in the incubator and wash off the sperm attached to the oocyte, and in vitro fertilization Cultivation is complete. In the present invention, the corresponding concentration of caffeic acid phenethyl ester and polyvinylpyrrolidone are combined in the freezing base solution I, and the synergistic effect of the two can effectively improve the vitality, plasma membrane integrity rate and DNA integrity rate of thawed pig sperm. Thus, the in vitro fertilization effect of thawed porcine sperm is improved.