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Streptococcus thermophilus freeze-drying protective agent and preparation method thereof

A technology of streptococcus thermophilus and freeze-drying protective agent, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of decreased activity of fermentation agents, damage of lactic acid bacteria cells or passivation of dead enzyme protein molecules , to achieve a long storage period, the survival rate is not easy to reduce, and improve the effect of freezing resistance

Active Publication Date: 2013-12-25
SHAANXI YATAI DAIRY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the freeze-drying process will inevitably cause certain damage or death to the lactic acid bacteria cells and the passivation of some enzyme protein molecules.
If the choice of protective agent is improper, the vigor of the starter will decrease accordingly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The composition of Streptococcus thermophilus lyoprotectant is: 10g skimmed milk powder, 3g raffinose, 4g soybean oligosaccharides, 1.5g dextran D40, 0.3g sodium tripolyphosphate, 0.006g propyl gallate, 100ml distilled water .

[0029] Among them, soybean oligosaccharides are commercially available from Rosenbow Technology Co., Ltd., and are light yellow powders that are easily soluble in water; dextran D40 is commercially available from Rosenbow Technology Co., Ltd., and are white powders that are easily soluble in water.

[0030] Its preparation method:

[0031] 1) Heat and dissolve the skim milk powder according to the amount, adjust the pH to 7.0 with NaOH solution, then sterilize at 110°C for 15 minutes, and cool to room temperature;

[0032] 2) Dissolve raffinose, soybean oligosaccharides and dextran D40 in distilled water according to the amount, then sterilize at 112°C for 12 minutes, and cool to room temperature;

[0033] 3) Weigh sodium tripolyphosphate and ...

Embodiment 2

[0036] The composition of Streptococcus thermophilus freeze-drying protection agent is: skim milk powder 13g, raffinose 5g, soybean oligosaccharide 5g, dextran D40 2.0g, sodium tripolyphosphate 0.4g, propyl gallate 0.005g, distilled water 100ml .

[0037] Its preparation method:

[0038] 1) Heat and dissolve the skim milk powder according to the amount, and adjust the pH to 6.9 with NaOH solution, then sterilize at 100°C for 14 minutes, and cool to room temperature;

[0039] 2) Dissolve raffinose, soybean oligosaccharides and dextran D40 in distilled water according to the amount, then sterilize at 100°C for 14 minutes, and cool to room temperature;

[0040] 3) Weigh sodium tripolyphosphate and propyl gallate according to the amount and dissolve them in sterile saline, then filter and sterilize with a 0.22 μm filter membrane; mix the damp heat sterilized solution with the above filter-sterilized solution according to quantity , to obtain the Streptococcus thermophilus lyopro...

Embodiment 3

[0043] The composition of Streptococcus thermophilus freeze-drying protection agent is: 14g of skimmed milk powder, 4g of raffinose, 6g of soybean oligosaccharides, 2.6g of dextran D40, 0.6g of sodium tripolyphosphate, 0.01g of propyl gallate, and 100ml of distilled water .

[0044] Its preparation method:

[0045] 1) Heat and dissolve the skim milk powder according to the amount, and adjust the pH to 6.9 with NaOH solution, then sterilize at 115°C for 10 minutes, and cool to room temperature;

[0046]2) Dissolve raffinose, soybean oligosaccharides and dextran D40 in distilled water according to the amount, then sterilize at 115°C for 10 minutes, and cool to room temperature;

[0047] 3) Weigh sodium tripolyphosphate and propyl gallate according to the amount and dissolve them in sterile saline, then filter and sterilize with a 0.22 μm filter membrane; mix the damp heat sterilized solution with the above filter-sterilized solution according to quantity , to obtain the Strept...

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Abstract

The invention discloses a streptococcus thermophilus freeze-drying protective agent and a preparation method thereof. The streptococcus thermophilus freeze-drying protective agent comprises the following components in parts by mass: 10-20 parts of dried skim milk, 3-10 parts of raffinose, 4-10 parts of soybean oligosaccharides, 1.05-5.0 parts of dextran D40, 0.3-0.8 parts of sodium tripolyphosphate, 0.005-0.02 parts of propylgallate and 100 parts of distilled water, wherein the soybean oligosaccharides and the dextran D40 can both be purchased from LUOSHENBO science and technology Ltd.. The anti-freezing ability of thallus can be increased by adding a freeze-drying protective agent during a thallus powder preparing process, thus the freeze-drying survival rate of the thallus powder can be increased, the storage period can be longer, and the survival rate can not be easily lowered.

Description

technical field [0001] The invention belongs to the technical field of preservation of streptococcus thermophilus, and relates to a freeze-drying protectant for streptococcus thermophilus and a preparation method thereof. Background technique [0002] In recent years, with the improvement of people's living standards and the enhancement of health awareness in our country, the output of yogurt has increased at an average rate of 25% per year, which puts forward new requirements for the quality, characteristics and types of yogurt starter. Streptococcus thermophilus is one of the commonly used lactic acid bacteria for the preparation of starter in yogurt production. The quality of yogurt is closely related to the quality of starter. [0003] At present, the preparation of Streptococcus thermophilus powder mainly adopts vacuum freeze-drying technology. Vacuum freeze-drying is a key technology for producing commercial lactic acid bacteria starter. It sublimates the microbial c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/46
Inventor 陈合李串娜舒国伟王勇
Owner SHAANXI YATAI DAIRY CO LTD
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