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Composite protective agent for increasing freeze-drying survival rate of lactobacillus plantarum and preparation method thereof

A technology of Lactobacillus plantarum and survival rate, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low number of viable bacteria and low survival rate of freeze-drying, achieve long storage period, improve freeze-drying Dry survival rate, effect of improving freezing resistance

Inactive Publication Date: 2016-03-23
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a method that can solve the problems of low freeze-drying survival rate and few viable counts of Lactobacillus plantarum powder in the freeze-drying process, and significantly improve the freeze-dried survival rate of plantarum lactobacillus and the viable count of bacterial powder. Composite protective agent and preparation method thereof

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The composition of Lactobacillus plantarum freeze-drying protection agent: comprises 6 parts of soybean polysaccharides, 6.5 parts of galactose, 8 parts of fructooligosaccharides, 6 parts of trehalose, 18 parts of skim milk, 4 parts of preserved acid and 100 parts distilled water.

[0022] The preparation method of protective agent is:

[0023] 1) Take 100 parts of distilled water according to the number of parts by mass and divide it into three parts, add 6 parts of soybean polysaccharides, 6.5 parts of galactose, and 8 parts of fructooligosaccharides to one of them: after 6 parts of trehalose dissolves, the Sterilize for 15 minutes, and obtain solution 1 after cooling;

[0024] 2) Add 18 parts of skim milk to another part of distilled water to dissolve to obtain a skim milk solution, and sterilize at 107°C for 15 minutes to obtain solution 2;

[0025] 3) Dissolve 4 parts of proline in the remaining distilled water, make a solution and sterilize it with a 0.22-micron...

Embodiment 2

[0030] The composition of Lactobacillus plantarum lyoprotectant: comprises 8 parts of soybean polysaccharides, 8 parts of galactose, 8 parts of fructo-oligosaccharides, 6 parts of trehalose, 18 parts of skim milk, 5 parts of preserved acid and 100 parts distilled water.

[0031] The preparation method of protective agent is:

[0032] 1) Divide 100 parts of distilled water into three parts according to the number of parts by mass, add 8 parts of soybean polysaccharide, 8 parts of galactose, 8 parts of fructooligosaccharide and 6 parts of trehalose to one part, and dissolve them at 110 ° C. Sterilize for 15 minutes, and obtain solution 1 after cooling;

[0033] 2) Add 18 parts of skim milk to another part of distilled water to dissolve to obtain a skim milk solution, and sterilize at 107°C for 15 minutes to obtain solution 2;

[0034] 3) Dissolve 5 parts of proline in the remaining distilled water, prepare a solution and sterilize it with a 0.22-micron filter membrane to obtai...

Embodiment 3

[0040] The composition of Lactobacillus plantarum freeze-drying protection agent: comprises 5 parts of soybean polysaccharides, 8 parts of galactose, 8 parts of fructooligosaccharides, 7 parts of trehalose, 120 parts of skim milk, 5.5 parts of preserved acid and 100 parts distilled water.

[0041] The preparation method of protective agent is:

[0042] 1) Divide 100 parts of distilled water into three parts according to the number of parts by mass, add 5 parts of soybean polysaccharide, 8 parts of galactose, 8 parts of fructooligosaccharide and 7 parts of trehalose to one part, and dissolve them at 110 ° C. Sterilize for 15 minutes, and obtain solution 1 after cooling;

[0043] 2) Add 20 parts of skim milk to another part of distilled water to dissolve to obtain a skim milk solution, and sterilize at 107°C for 15 minutes to obtain solution 2;

[0044] 3) Dissolve 5.5 parts of proline in the remaining distilled water, prepare a solution and filter and sterilize with a 0.22 mi...

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Abstract

The invention provides a composite protective agent for increasing the freeze-drying survival rate of lactobacillus plantarum and a preparation method thereof. The protective agent is prepared from soybean polysaccharide, galactose, fructo-oligosaccharide, trehalose, skimmed milk, proline and distilled water. The preparation method includes the steps that the distilled water is divided into three parts, and soybean polysaccharide, galactose, fructo-oligosaccharide and trehalose are added to one part for dissolution and sterilization; the skimmed milk is added to another part of the distilled water for dissolution, and a skimmed milk solution is obtained and sterilized; proline is dissolved in the remaining distilled water, and membrane filtration sterilization is conducted; the three prepared solutions are evenly mixed under the sterile condition, and then the composite freeze-drying protective agent is obtained. According to the freeze-drying protective agent for lactobacillus plantarum, a freeze-drying protective additive is added in the fungus powder preparation process, the anti-freezing capacity of fungi can be improved, and therefore the freeze-drying survival rate of the fungus powder is increased, the storage period is long, and the survival rate is not likely to be reduced. The freeze-drying survival rate of the prepared fungus powder of lactobacillus plantarum ranges from 82.73% to 93.26%, and the viable count of the fungus powder ranges from 1.14*10<11> cfu / g to 1.98*10<11> cfu / g.

Description

technical field [0001] The invention belongs to the technical field of production of Lactobacillus plantarum products, and in particular relates to a compound protective agent for improving the freeze-dried survival rate of Lactobacillus plantarum and a preparation method thereof. Background technique [0002] With the improvement of people's living standards, the consumption of probiotic food is increasing year by year. The main way to add probiotics to food is to add bacteria directly or in the form of probiotic preparations. More and more probiotics are used to replace the function of antibiotics. Among them, lactic acid bacteria (Lactic Acid Bacteria, LAB) are important probiotics for the human body. They can regulate the normal flora of the human gastrointestinal tract, maintain micro-ecological balance, improve food digestibility and biological value, reduce serum cholesterol, and inhibit the growth and reproduction of spoilage bacteria in the intestinal tract. and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/25
CPCC12N1/04
Inventor 陈合王帅舒国伟陈立
Owner SHAANXI UNIV OF SCI & TECH
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