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Lactobacillus acidophilus anti-freeze culture medium and application method thereof

A technology of Lactobacillus acidophilus and culture medium, which is applied in the field of antifreeze culture medium for improving the freeze-drying survival rate of Lactobacillus acidophilus and the viable count of bacteria powder, and can solve the problems of low viable count and low freeze-drying survival rate. , to achieve the effect of high anti-freezing ability

Inactive Publication Date: 2012-04-11
SHAANXI RES INST OF AGRI PRODS PROCESSING TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of Lactobacillus acidophilus antifreeze culture medium and its application method, which solves the problem that the freeze-drying survival rate of Lactobacillus acidophilus in the freeze-drying process is not high and the number of viable bacteria is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Accurately weigh 18g glucose, 0.5g Tribulus terrestris polysaccharide, 3.5g yeast extract, 5.0g sodium chloride, 9.0g soybean peptone, 1.5g diammonium hydrogen citrate, 1.5g dipotassium hydrogen phosphate, 0.1g MgSO 4 Dissolve 0.8ml Tween 80 in 950mL distilled water, heat to dissolve and mix well, adjust the pH to 5.6 with 1mol / L NaOH, then put it in an anaerobic bottle, the filling volume is 90%, and put it on the rubber stopper of the anaerobic bottle cap Insert a 1ml syringe needle, sterilize at 118°C for 15 minutes, pull out the syringe needle after sterilization, and cool to room temperature to obtain medium A;

[0021] The preparation method of the described Tribulus terrestris polysaccharide comprises the following steps: crush the dried Tribulus terrestris and sieve it through a 40-mesh sieve to obtain the Tribulus terrestris powder, add 1400mL of distilled water to 100g of the Tribulus terrestris powder, adjust the pH value to 4.8, then add the Tribulus terrestr...

Embodiment 2

[0026] Accurately weigh 20g glucose, 0.5g tribulus polysaccharide, 4.5g yeast extract, 4.0g sodium chloride, 8.0g soybean peptone, 2.0g diammonium hydrogen citrate, 1.5g dipotassium hydrogen phosphate, 0.1g MgSO 4 Dissolve 1.0ml of Tween 80 in 950mL of distilled water, heat to dissolve and mix well, adjust the pH to 6.0 with 1mol / L NaOH, then put it in an anaerobic bottle, the filling volume is 90%, and put it on the rubber stopper of the anaerobic bottle cap Insert a 1ml syringe needle, sterilize at 118°C for 15 minutes, pull out the syringe needle after sterilization, and cool to room temperature to obtain medium A;

[0027] The preparation method of the Tribulus terrestris polysaccharide, wherein, the temperature of the water bath insulation is 55°C, the drying temperature of the wet polysaccharide is 55°C, and other steps are the same as in Example 1;

[0028] Accurately weigh 0.5g of betaine, 0.4g of mannitol and 1.2g of sodium glutamate and dissolve them in 50ml of distill...

Embodiment 3

[0032] Accurately weigh 19g glucose, 0.4g tribulus polysaccharide, 5.0g yeast extract, 6.0g sodium chloride, 12.0g soybean peptone, 0.5g diammonium hydrogen citrate, 1.0g dipotassium hydrogen phosphate, 0.2g MgSO 4 Dissolve 0.5ml Tween 80 in 950mL distilled water, heat to dissolve and mix evenly, adjust the pH to 5.8 with 1mol / L NaOH, then put it in an anaerobic bottle, the filling volume is 90%, and put it on the rubber stopper of the anaerobic bottle cap Insert a 1ml syringe needle, sterilize at 120°C for 15 minutes, pull out the syringe needle after sterilization, and cool to room temperature to obtain medium A;

[0033] The preparation method of the Tribulus terrestris polysaccharide, wherein, the temperature of the water bath insulation is 50°C, the drying temperature of the wet polysaccharide is 50°C, and other steps are the same as in Example 1;

[0034] Accurately weigh 0.3g of betaine, 0.3g of mannitol and 0.8g of sodium glutamate and dissolve them in 50ml of distille...

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Abstract

The invention provides a lactobacillus acidophilus anti-freeze culture medium and an application method thereof. The culture medium includes the following components by weight: 16-20 g of glucose, 0.4-0.8 g of fructus tribuli polysaccharide, 3-5 g of yeast extract, 4-6 g of sodium chloride, 0.3-0.5 g of mannitol, 0.8-1.2 g of sodium glutamate, 8-12 g of soybean peptone, 0.5-2.0 g of diammonium hydrogen citrate, 0.2-0.5 g of betaine, 1.0-2.5 g of dipotassium phosphate, 0.1-0.2 g of MgSO4, 0.5-1.0 ml of Tween-80 and 1,000 ml of distilled water. The culture medium has the advantages that: after lactobacillus acidophilus is cultured by using the anti-freeze culture medium, the freeze-dry survival rate of the lactobacillus acidophilus can reach up to 79.54 percent, and viable count of fungal powder reaches up to 4.09*10<11> cfu / g; and the lactobacillus acidophilus MRS (de Man, Rogosa and Sharp) broth is used for culture, so that the freeze-dry survival rate of the prepared fungal powder isonly 49.72 percent, and the viable count is only 1.24*10<11> cfu / g.

Description

technical field [0001] The invention belongs to the technical field of production of Lactobacillus acidophilus products, and relates to an antifreeze medium for improving the freeze-dried survival rate of Lactobacillus acidophilus and the number of live bacteria in bacterial powder and an application method thereof. Background technique [0002] Lactobacillus acidophilus (Lactobacillus acidophilus) is one of the main microorganisms in the intestinal tract of humans and animals, and it is also a probiotic in the lactic acid bacteria family that attaches great importance to research and development. When there is a certain amount, it can effectively regulate the balance between beneficial and harmful microbial flora in the intestinal tract, so as to achieve the effect of promoting the health of the host. A large number of scientific studies have proved that Lactobacillus acidophilus can: ① relieve lactose intolerance; ② inhibit pathogenic bacteria in the intestinal tract and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/23
Inventor 舒国伟王娟陈合马齐秦涛张华蔡宁冈
Owner SHAANXI RES INST OF AGRI PRODS PROCESSING TECH
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