Microbial agent for extracting herbal fibers as well as preparation method and application of microbial agent

A technology of microbial inoculum and herbal fiber, which is applied in the methods of using microorganisms, using microorganisms/enzyme cellulose to treat, and preserving microorganisms, etc., can solve the problems of difficult preparation of strains, easy degradation of strains, and expanded application of variation, etc. To achieve the effect of improving the anti-freezing ability, the theoretical and practical significance, and the stability enhancement

Active Publication Date: 2022-06-24
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the DCE-01 strain is a heat-sensitive Gram-negative bacterium, and its strain preparation technology is relatively difficult, especially the technical problems that the strain is easy to degrade and mutate, which restricts its further expanded application in the biological degumming of hemp

Method used

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  • Microbial agent for extracting herbal fibers as well as preparation method and application of microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 A kind of preparation method of microbial inoculum for fiber extraction

[0047]Pick a ring of cold-acclimated DCE-01 strains from the slanted stock moss preserved in the Hemp Institute of the Chinese Academy of Agricultural Sciences and inoculate it into 5 mL of improved broth (nutrient broth), fully suspend and incubate at 35°C 6h; after dilution, spread on YQ nutrient agar plate, cultivate at 35°C for 20h, and isolate single colonies; select typical colonies to streak on a fresh slant, incubate at 35°C for 20h, vacuum pack, and store at room temperature; put 1mL of sterilized modified broth The culture solution (nutrient broth culture solution) is poured into one of the above-mentioned slanted bacterial species, and the surface bacterial moss is gently scraped with an inoculation ring to ensure that all the bacterial moss is mixed into the culture solution.

[0048] Specifically, the mass ratio formula of the above-mentioned nutrient broth culture soluti...

Embodiment 2

[0058] Embodiment 2 A kind of preparation method of microbial inoculum for fiber extraction

[0059] Pick a ring of cold-acclimated DCE-01 strains from the slanted stock moss preserved in the Hemp Institute of the Chinese Academy of Agricultural Sciences and inoculate it into 5 mL of improved broth (nutrient broth), fully suspend and incubate at 35°C 6h; after dilution, spread on YQ nutrient agar plate, cultivate at 35°C for 20h, and isolate single colonies; select typical colonies to streak on a fresh slant, incubate at 35°C for 20h, vacuum pack, and store at room temperature; put 1mL of sterilized modified broth The culture solution (nutrient broth culture solution) is poured into one of the above-mentioned slanted bacterial species, and the surface bacterial moss is gently scraped with an inoculation ring to ensure that all the bacterial moss is mixed into the culture solution.

[0060] Specifically, the mass ratio formula of the above-mentioned nutrient broth culture solut...

Embodiment 3

[0070] Embodiment 3 A kind of preparation method of microbial inoculum for fiber extraction

[0071] Pick a ring of cold-acclimated DCE-01 strains from the slanted stock moss preserved in the Hemp Institute of the Chinese Academy of Agricultural Sciences and inoculate it into 5 mL of improved broth (nutrient broth), fully suspend and incubate at 35°C 6h; after dilution, spread on YQ nutrient agar plate, cultivate at 35°C for 20h, and isolate single colonies; select typical colonies to streak on a fresh slant, incubate at 35°C for 20h, vacuum pack, and store at room temperature; put 1mL of sterilized modified broth The culture solution (nutrient broth culture solution) is poured into one of the above-mentioned slanted bacterial species, and the surface bacterial moss is gently scraped with an inoculation ring to ensure that all the bacterial moss is mixed into the culture solution.

[0072] Specifically, the mass ratio formula of the above-mentioned nutrient broth culture solut...

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Abstract

The invention discloses a microbial agent for herbal fiber extraction and a preparation method and application thereof, and the preparation method comprises the following steps: inoculating an activated DCE-01 strain into an improved fermentation culture solution for expanding culture and fermentation, then concentrating the obtained mature fermentation solution, finally adding a protective agent, uniformly mixing, and then drying the obtained bacterial suspension. The improved fermentation culture solution is a fermentation culture solution containing 0.08 to 0.25 weight percent of mannose, 0.075 to 0.24 weight percent of trehalose and 0.2 to 0.55 weight percent of glucose. The formula of the improved fermentation culture solution is adjusted, mannose and trehalose are adopted to replace part of glucose to prepare the improved fermentation culture solution, the stability of macromolecules in cells is enhanced after thalli suck mannose and trehalose, and the freezing resistance of the thalli is improved; the composite protective agent containing saccharides, proteins and starch is selected, so that the bacterial adsorption capacity is large, the protective effect is good, and the practical application effect is excellent.

Description

technical field [0001] The invention belongs to the technical field of treatment of herbal fiber raw materials, and more particularly relates to a microbial inoculum for herbal fiber extraction and a preparation method and application thereof. Background technique [0002] Herbal fiber is one of the most important natural fiber sources in the world; in addition to a large amount of cellulose fiber, the raw material of herbal fiber also contains "impurities" such as pectin, hemicellulose and lignin. In order to obtain pure fiber from herbal fiber raw materials, all non-cellulosic substances of bast fiber raw materials must be removed by "cleaning"; therefore, fiber extraction is the basic and key link in the processing of herbal fiber raw materials. [0003] Biological extraction method is favored because of its advantages of "green, high efficiency and energy saving". [0004] The core of herbal fiber biological extraction is high-efficiency strains; a series of strains rel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12N1/36D21C5/00
CPCC12N1/20C12N1/04C12N1/36D21C5/005
Inventor 成莉凤段盛文冯湘沅杨琦郑科彭正红
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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