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A method for detecting pesticide residues in Chinese medicinal materials

A technology for pesticide residues and Chinese medicinal materials, applied in the field of pesticide detection, can solve the problems of inconsistent standards, poor repeatability, complex Chinese medicinal materials matrix, etc., and achieve high accuracy and repeatability.

Active Publication Date: 2022-06-14
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to the national standard 2763 and the Chinese Pharmacopoeia 2015, my country has set maximum residues for about 600 kinds of pesticides in agricultural products, but among Chinese medicinal materials, only 4 kinds of Chinese medicinal materials have stipulated a total of 7 kinds of pesticides mainly organochlorine pesticides Maximum residue limits, which lead to inconsistent standards between exporting countries and importing countries in the process of exporting traditional Chinese medicine, which has become a stumbling block for Chinese medicine to go global
[0004] The matrix of traditional Chinese medicinal materials is relatively complex, and the multi-residue detection of pesticides has the defects of low accuracy and poor repeatability

Method used

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  • A method for detecting pesticide residues in Chinese medicinal materials
  • A method for detecting pesticide residues in Chinese medicinal materials
  • A method for detecting pesticide residues in Chinese medicinal materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] The Chinese medicinal material to be tested (Ophiopogon japonicus) was pulverized with a high-speed pulverizer and passed through a 40-mesh sieve for use. Accurately weigh 2g of Chinese herbal medicine powder, add 10mL of water, vortex for 1min to obtain the first extraction product, add 6mL of 0.75% acetic acid acetonitrile, vortex for 1min, and ultrasonically extract for 15min to obtain the second extraction product, add 6g of anhydrous magnesium sulfate And 1.5g sodium chloride, shake vigorously for 1min. Centrifuge at 8000rpm for 5 minutes, use the supernatant as the matrix extract, take 1mL of the matrix extract, add 150mg anhydrous magnesium sulfate and 5mg nano-zinc oxide, vortex for 1min, centrifuge at 11000rpm for 5 minutes, take the supernatant and pass through a 0.22μm filter membrane Then add the sample vial as the liquid to be tested.

Embodiment 2

[0072] The Chinese medicinal material to be tested (Ophiopogon japonicus) was pulverized with a high-speed pulverizer and passed through a 40-mesh sieve for use. Accurately weigh 2 g of Chinese medicinal powder, add 5 mL of water, vortex for 1 min, add 10 mL of 0.75% acetic acid acetonitrile, vortex for 1 min, ultrasonically extract for 15 min, add 6 g of anhydrous magnesium sulfate and 1.5 g of sodium chloride, and shake vigorously for 1 min. Centrifuge at 8000rpm for 5 minutes, use the supernatant as the matrix extract, take 1mL of the matrix extract, add 150mg anhydrous magnesium sulfate and 5mg nano-zinc oxide, vortex for 1min, centrifuge at 11000rpm for 5 minutes, take the supernatant and pass through a 0.22μm filter membrane Then add the sample vial as the liquid to be tested.

Embodiment 3

[0074] The Chinese herbal medicine to be tested (Yuzhu) was pulverized with a high-speed pulverizer and passed through a 40-mesh sieve for use. Accurately weigh 2g of Chinese herbal medicine powder, add 10mL of water, vortex for 1min to obtain the first extraction product, add 6mL of 0.75% acetic acid acetonitrile, vortex for 1min, and ultrasonically extract for 15min to obtain the second extraction product, add 6g of anhydrous magnesium sulfate And 1.5g sodium chloride, shake vigorously for 1min. Centrifuge at 8000rpm for 5 minutes, use the supernatant as the matrix extract, take 1mL of the matrix extract, add 150mg anhydrous magnesium sulfate and 5mg nano-zinc oxide, vortex for 1min, centrifuge at 11000rpm for 5 minutes, take the supernatant and pass through a 0.22μm filter membrane Then add the sample vial as the liquid to be tested.

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Abstract

The disclosure provides a method for detecting pesticide residues in Chinese herbal medicines, and relates to the field of pesticide detection. The pulverized Chinese herbal medicines to be tested are mixed with water and subjected to a first extraction to obtain a first extraction product; the first extraction product is mixed with acetic acid Acetonitrile aqueous solution is mixed and subjected to the second extraction to obtain the second extraction product; the second extraction product is mixed with anhydrous magnesium sulfate and sodium chloride and subjected to the third extraction to obtain the third extract; after the third extract is centrifuged Obtain the supernatant as the matrix extract, mix the matrix extract with the purification agent and perform purification treatment, and then centrifuge the purified material as the test solution; use ultra-high performance liquid chromatography-tandem mass spectrometry to test liquid is measured. The detection method can realize the simultaneous detection of up to 76 kinds of pesticides including insecticides, fungicides and plant growth regulators in Chinese herbal medicines, and has high accuracy and repeatability.

Description

technical field [0001] The present disclosure relates to the field of pesticide detection, in particular, to a method for detecting pesticide residues in Chinese medicinal materials. Background technique [0002] Traditional Chinese medicine is one of the most important cultural heritages in my country, and has the functions of health care, prevention and treatment of diseases. Today, with the development of modern science and technology, traditional Chinese medicine is gradually entering the world. In recent years, the planting area of ​​traditional Chinese medicine in China has grown rapidly. There are more than 430 planting bases of common varieties, and the planting area is about 3.067 million hectares. 2 . In recent years, people have paid more and more attention to the safety of traditional Chinese medicine, because traditional Chinese medicine must not only ensure its efficacy, but also ensure its safety. Some studies have pointed out that about 65-80% of people pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/062
Inventor 戴小枫范蓓孔志强李敏敏李瑞瑆金诺陈捷胤孙玉凤刘佳萌孙晶贾宁
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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