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A kind of Aspergillus fumigatus strain and its application in degrading polyvinyl alcohol

A technology of Aspergillus fumigatus and polyvinyl alcohol, applied in the field of microorganisms, can solve the problems of low strain efficiency, unable to meet the needs of application, etc., and achieve the effect of strong carbon source utilization ability

Active Publication Date: 2021-10-08
广东省科学院生物与医学工程研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since Nord first reported that Fusarium can degrade PVA in 1936, some PVA degrading bacteria have been isolated and purified, such as Pseudomonas O-3, Pseudomonas vesiculars PD, Pseudomonas putida, Bacillus magaterium, Acinetiobactor sp., Xanthamonas sp., etc., but , the vast majority of strains still cannot meet the needs of practical applications due to low efficiency and other reasons

Method used

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  • A kind of Aspergillus fumigatus strain and its application in degrading polyvinyl alcohol
  • A kind of Aspergillus fumigatus strain and its application in degrading polyvinyl alcohol
  • A kind of Aspergillus fumigatus strain and its application in degrading polyvinyl alcohol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Screening of a PVA degrading functional strain

[0027] 1.1 Make an inorganic salt medium plate containing PVA as the only carbon source:

[0028] Medium consists of the following components: K 2 HPO 4 0.7g, KH 2 PO 4 0.7g, MgSO 4 ·7H 2 O 0.7g, NaCl 0.005g, FeSO 4 ·7H 2 O 0.002g, ZnSO 4 ·7H 2 O 0.002g, MnSO 4 ·H 2 O 0.001g, PVA 10g, agar 18g, distilled water 1000mL. The culture medium was sterilized at 121°C for 20 minutes, then cooled until it was not hot to the hands, and poured into a plate in a sterile operating table.

[0029] 1.2 Cultivate PVA-degrading functional bacteria:

[0030] Wash the plastic bags with degradation characteristics in sterile water to obtain a cleaning solution containing microorganisms, and spread it on an inorganic salt medium plate with PVA as the only carbon source, and cultivate it at 30°C to obtain PVA-degrading functional bacteria.

[0031] 1.3 Isolation of a single colony of PVA-degrading functional strains: ...

Embodiment 2

[0035] Morphology, carbon source utilization, molecular biology identification of embodiment 2 bacterial strain GPVA1

[0036]The detection and identification of the strain GPVA1 was entrusted to the China Center for Type Culture Collection, and the results are as follows:

[0037] 2.1 Morphological features

[0038] The strain grew well on the PDA medium. After being cultured at 28°C for 3 days, the colony protruded to the surface of the medium, loose, dry, fluffy, white at the edge, and smoke green in the middle ( figure 1 ), a large number of spores are produced, the spores are spherical, the apical capsule is in the shape of an inverted flask, and no pigment is produced ( figure 2 ).

[0039] 2.2 Characteristics of carbon source utilization

[0040] The utilization of 95 carbon sources by GPVA1 was detected by biolog FF plate, and the results showed that it could utilize 80 of them.

[0041] Table 1 Detection of carbon source utilization of GPVA1 strain

[0042]

...

Embodiment 3

[0052] Example 3 bacterial strain GPVA1 degrades PVA in plate medium

[0053] Make an inorganic salt medium plate containing PVA as the only carbon source. Medium consists of the following components: K 2 HPO 4 0.7g, KH 2 PO 4 0.7g, MgSO 4 ·7H 2 O 0.7g, NaCl 0.005g, FeSO 4 ·7H 2 O 0.002g, ZnSO 4 ·7H 2 O 0.002g, MnSO 4 ·H 2 O 0.001g, PVA 10g, agar 18g, distilled water 1000mL. The culture medium was sterilized at 121°C for 20 minutes, then cooled until it was not hot to the hands, and poured into a plate in a sterile operating table. After inoculating GPVA1 on the plate, it was incubated at 30°C. After the colonies spread all over the plate, take a small piece of culture medium with hyphae, transfer it to a new medium plate, and culture it at 30°C for 2 days. Spray boric acid and potassium iodide-iodine solution into the culture medium with a watering can to form a blue-green complex with PVA in the culture medium to confirm the presence of PVA.

[0054] The res...

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Abstract

The invention discloses an Aspergillus fumigatus strain and its application in degrading polyvinyl alcohol. The Aspergillus fumigatus strain is named Aspergillus fumigatus (Aspergillus fumigatus) GPVA1, the preservation number is CCTCC NO:M 2020213, and the preservation date is June 2020 17th. The present invention provides for the first time the Aspergillus fumigatus bacterial strain capable of degrading polyvinyl alcohol (PVA), which can grow and degrade PVA in solid inorganic salt medium or liquid inorganic salt medium with polyvinyl alcohol as the only carbon source, at 24 The concentration of PVA in the liquid medium can be reduced by 2.72g / L within an hour, and it has the potential to be applied to the treatment of PVA-contaminated microorganisms in the environment. The aspergillus fumigatus strain provided by the invention not only has the ability to degrade polyvinyl alcohol, but also can utilize at least 80 kinds of carbon sources, and has strong carbon source utilization ability.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an Aspergillus fumigatus strain and its application in degrading polyvinyl alcohol. Background technique [0002] Polyvinyl alcohol (PVA) is a chemical raw material made from petroleum cracking products. It has good water solubility, adhesion, size film toughness and abrasion resistance, and strong organic solvent tolerance. It is widely used in textiles, paper Product manufacturing, food packaging and medical equipment and other industries. PVA is the water-soluble polymer with the largest production volume, and its production and consumption are increasing year by year, resulting in a large amount of PVA being discharged into the environment, causing environmental pollution and affecting ecological balance. PVA has a large surface activity and can form a large amount of foam in water, resulting in oxygen-enriched water and increased viscosity, thereby inhibiting or even...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C02F3/34C12R1/68C02F101/34
CPCC12N1/14C02F3/347C02F2101/34C12R2001/68C12N1/145
Inventor 龙碧波陈骏佳谢东高旭华杨翀李圆王珂赵阳刘海露黄瑶珠
Owner 广东省科学院生物与医学工程研究所
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