Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting D-pinitol in soybeans

A detection method and pinitol technology, applied in measuring devices, instruments, scientific instruments, etc., to achieve the effects of good separation effect, wide linear range and high sensitivity

Pending Publication Date: 2020-12-22
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] D-pinitol is different from D-ribose and inositol in nature. Therefore, IC-PAD has not yet been used to detect D-pinitol in soybeans.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting D-pinitol in soybeans
  • Method for detecting D-pinitol in soybeans
  • Method for detecting D-pinitol in soybeans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the detection method of D-pinitol in a kind of soybean, concrete implementation steps are as follows:

[0043] 1), prepare D-pinitol standard solution and carry out ion chromatography-pulse amperometric detection (IC-PAD).

[0044] Accurately weigh 100mg of D-pinitol standard substance and place it in a 100mL volumetric flask, and use deionized water to ultrasonically dissolve and set the volume to the mark as a standard stock solution; Pinitol standard solution concentration of 0.1mg / L, 0.5mg / L, 1.0mg / L, 5.0mg / L, 10.0mg / L, 20.0mg / L, 50.0mg / L, 100mg / L 8 standard working solutions .

[0045] The above-mentioned standard solutions (standard working solutions) of different concentrations were respectively subjected to ion chromatography-pulse amperometric detection, and obtained standard chromatograms, chromatographic conditions:

[0046] (1) Chromatographic column: Thermo Scientific CarboPac MA1 guard column (50mm×4mm i.d.) + analytical column (250mm×4mm i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting D-pinitol in soybeans, which comprises the following steps: preparing a working solution from a D-pinitol standard substance, and obtaining a D-pinitol standard chromatogram by an ion chromatography-pulse ampere detection method; drawing a relation curve graph of the concentration of the D-pinitol and the chromatographic peak area; pretreating a soybean sample to obtain a to-be-detected sample solution; detecting the to-be-detected sample solution according to the ion chromatography-pulse ampere detection method to obtain the peak area of the D-pinitol; substituting the peak area into the relation curve graph to obtain the concentration of the D-pinitol in the to-be-detected sample solution, and finally obtaining the concentration of the D-pinitol in the soybeans. The method for detecting the D-pinitol in the soybeans is good in repeatability and high in sensitivity.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and instrumental analysis, and in particular relates to a detection method for D-pinitol in soybeans. Background technique [0002] D-pinitol, also known as dextro-inositol methyl ether, methyl inositol, keninol, behenyl alcohol, 3-methyl inositol, pinetol, is the 3-position monomethyl etherification product of polycyclic inositol An isomer of pine, which is widely present in the flowers and leaves of pine plants, beans, alfalfa and many plants. D-pinitol has an insulin-like effect, can reduce and regulate the body's blood sugar balance, and relieve insulin resistance. It is a new type of hypoglycemic functional substance. In addition, D-pinitol also has antitussive and expectorant effects, as well as anti-inflammatory and anti-edema activities, and can promote the body's absorption of creatine. In recent years, it has been widely used as a pharmaceutical raw material and health product...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/64
CPCG01N30/02G01N30/06G01N30/14G01N30/64
Inventor 朱作艺王伟张玉王君虹李雪孙素玲
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com