Preparation method of Miltenberger blood group series phenotype red blood cells

Abstract

The invention discloses a preparation method of Miltenberger blood group series phenotype red blood cells. The preparation method includes the following steps: step one, adopting monoclonal antibodiesto identify common clinically significant Rh, Kidd, MNS, Duffy, Lewis and P system antigens on Miltenberger blood group positive type O red blood cells; step two, respectively performing PCR-SSP andgene sequencing validation on dose effect antigen C, c, E, e, M, N, S, s, Fya, Fyb, Jka and Jkb to obtain a red blood cell surface antigen pattern; step three, using a red blood cell reagent preservation solution to prepare into a Miltenberger blood group series phenotype red blood cell reagent; and step four, using the prepared Miltenberger blood group series phenotype red blood cell reagent andan existing irregular antibody screening cell reagent to parallelly detect the serum of a hospitalized blood transfusion patient, vertifying the diagnostic performance of the cell reagent, and obtaining a final product. Through the cooperation of the prepared Miltenberger blood group series phenotype red blood cells and the existing screening cells, detection can be performed on Miltenberger bloodgroup related antibodies, and therefore, safe blood transfusion can be guaranteed.

Description

technical field [0001] The invention relates to the technical field of preparation of erythrocyte products, in particular to a preparation method of erythrocytes of Miltenberger blood type series phenotype. Background technique [0002] Miltenberger blood group system antibody positive frequency is second only to Rh blood group system, can cause hemolytic transfusion reaction (hemolytic transfusion reaction, HTR) and newborn hemolytic disease (haemolytic disease of the newborn, HDN), is a clinically significant irregular antibody. Screening and identification of irregular antibodies in serum of patients with repeated blood transfusions found that anti-Mur accounted for 30.85% of all irregular antibodies. Anti-Mur can lead to acute and delayed HTR and severe HDN, there have been case reports at home and abroad. It can be seen that screening for Miltenberger blood group-related antibodies before clinical blood transfusion is of great significance to the safety of patients. ...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of preparation method of Miltenberger blood group phenotype erythrocytes, to solve the problem that there is no commercialized Miltenberger blood group phenotype red blood cells in the country proposed in the above-mentioned background technology, can not detect the relevant antibody of Miltenberger blood group in patient's serum, exists The problem of missed detection of antibodies of this blood group and the risk of hemolytic transfusion reaction caused by blood transfusion

Images