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A method for distinguishing Hei Mao Fu tea and Eucommia Fu tea based on Fu tea metabolomics

A technology of metabolomics and black hairy tea, applied in the field of analytical chemistry, achieves the effects of less sample consumption, simple and fast operation, and accurate and reliable results

Active Publication Date: 2022-06-21
陕西茯泽园茯茶有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The functions and sensory qualities of Fu tea made from different raw materials are quite different

Method used

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  • A method for distinguishing Hei Mao Fu tea and Eucommia Fu tea based on Fu tea metabolomics
  • A method for distinguishing Hei Mao Fu tea and Eucommia Fu tea based on Fu tea metabolomics

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Experimental program
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Effect test

Embodiment

[0017] The contents of kaempferol and rutin in Fucha tea samples were determined by ultra-high performance liquid chromatography-quadrupole / electrostatic field orbital hydrazine high-resolution mass spectrometry (UHPLC-Q ExactiveMS).

[0018] The samples to be tested include 4 samples of Hei Mao Fu tea made from Anhua dark tea, numbered T-1, T-2, T-3, and T-4 respectively, made from Eucommia ulmoides leaves from Lueyang, Shaanxi Province. Four Eucommia fucha samples were numbered DZ-1, DZ-2, DZ-3, and DZ-4 respectively.

[0019] The sample analysis steps are as follows:

[0020] 1. Fu tea sample collection:

[0021] Hei Mao Fu tea made from Anhua dark tea as raw material, Eucommia fucha tea made from Eucommia ulmoides leaves in Lueyang, Shaanxi Province (fucha tea processed under the same processing conditions), stored in -80 ℃ refrigerator.

[0022] 2. Determination method of kaempferol and rutin:

[0023] The tea samples were taken out from the -80 ℃ refrigerator, placed ...

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Abstract

The invention discloses a method for distinguishing Hei Mao Fu tea and Eucommia Fu tea based on the metabolomics of Fu tea. The technical scheme of the present invention is: for Fucha samples with unknown raw materials, use the ratio of kaempferol and rutin content in Fucha to discriminate Fucha samples in metabolomics research, and the ratio of kaempferol and rutin in the samples to be tested is above 0.51 The ones with the ratio of kaempferol and rutin below 0.06 are the samples of eucommia fucha. The invention has simple operation and reliable detection results, and can provide reference for the analysis of the metabolic characteristics of Fu tea and related Fu tea metabolomics research.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a method for distinguishing Hei Mao Fu tea and Eucommia fucha tea based on Fu tea metabolomics. Background technique [0002] As an important part of systems biology, metabolomics can detect multiple endogenous metabolites simultaneously. Metabolomics is increasingly used in tea related research, mainly for chemical analysis of tea species, origin, and processing. Fu tea is rich in ingredients. Long-term drinking of Fu tea can enhance human body constitution and delay aging. Fu tea made from different raw materials has great differences in function and sensory quality. Black hair tea is the traditional raw material for making Fu tea, and Eucommia fucha tea, as a new type of health-care tea, is deeply loved by the masses. [0003] Poria tea contains a large number of bioactive components such as flavonoids. Flavonoids are generated from glucose through the shikimic acid pathwa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8675
Inventor 赵燕妮陈丹李悦任嘉敏张坤曾乔秦俊哲胡歆梁艳
Owner 陕西茯泽园茯茶有限公司
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