Chinese herbaceous peony extract and preparation method and application thereof
A technology of extract and peony, applied in the field of peony extract and its preparation, can solve the problems of low extraction rate, difficult purification, complicated process, etc., achieve high extraction rate, keep bright and elastic, and take a short time
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[0028] The invention provides a preparation method of Paeoniae officinalis extract, comprising the steps of:
[0029] Taking peony root and pulverizing it to prepare peony root powder; the particle size of the peony root powder is not more than 850 μm;
[0030] extracting the peony root powder with a methanol aqueous solution with a volume concentration of 45-55%, and concentrating the obtained extract to prepare the first extract; the extraction method is heating to 30-75° C. for reflux extraction for 30-150 minutes;
[0031] adding ethyl acetate to the first extract for extraction, collecting the ethyl acetate phase and concentrating to dryness to prepare a second extract;
[0032] After the second extract is reconstituted, methanol is used as the eluent for gel elution, and the eluate containing the marker is detected and collected; the marker is pentagalloyl glucose.
[0033] Understandably, "gel elution" refers to elution with a gel column. "Concentrating the resulting ...
Embodiment 1
[0049] This embodiment provides a preparation method of Paeoniae officinalis extract, the steps are as follows:
[0050] (1) get Radix Paeoniae Alba, pulverize, cross 200 mesh sieves, obtain Radix Paeoniae Alba powder;
[0051](2) Take 5 g of Paeoniae officinalis root powder obtained in step (1), add 250 mL of methanol aqueous solution (volume concentration is 50%), and condense and reflux for extraction at a temperature of 70 ° C for 60 min; the obtained extract is mixed and cooled, 4500 r / min Centrifuge for 20 minutes, and filter under reduced pressure with filter paper with a pore size of 15 μm; the resulting filtrate is concentrated and evaporated to methanol, and the remaining liquid is extracted with ethyl acetate, and the ethyl acetate layer is collected, and the extraction is repeated until the ethyl acetate layer is colorless; the obtained ethyl acetate phase is concentrated to dryness to obtain a residue;
[0052] (3) Redissolve the residue of step (2) with methanol...
Embodiment 2
[0055] This embodiment provides a method for preparing a peony extract, the steps of which are the same as those in Embodiment 1, the main difference being that in step (2), the volume concentration of methanol in the methanol aqueous solution is different, which is 45%.
[0056] Specific steps are as follows:
[0057] (1) get Radix Paeoniae Alba, pulverize, cross 200 mesh sieves, obtain Radix Paeoniae Alba powder;
[0058] (2) Take 5 g of Paeoniae officinalis root powder obtained in step (1), add 250 mL of methanol aqueous solution (volume concentration is 45%), and condense and reflux for extraction at a temperature of 70 ° C for 60 min; the obtained extract is mixed, cooled, and 4500 r / min Centrifuge for 20 minutes, and filter under reduced pressure with filter paper with a pore size of 15 μm; the resulting filtrate is concentrated and evaporated to methanol, and the remaining liquid is extracted with ethyl acetate, and the ethyl acetate layer is collected, and the extracti...
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