Detection method of HLA-B*1502 gene, detection kit and application thereof

A technology for HLA-B and detection reagents, applied in biochemical equipment and methods, microbial measurement/testing, recombinant DNA technology, etc., can solve problems such as time-consuming, high cost, and cumbersome operations
CN112481374AActive Publication Date: 2021-03-12上海恩元生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
上海恩元生物科技有限公司
Publication Date
2021-03-12

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Abstract

The invention belongs to the technical field of biology, and provides a reagent for detecting human leukocyte antigen B site 1502 genotype (HLA-B*1502), application of the reagent in preparation of akit for guiding administration of antiepileptic drugs such as carbamazepine, oxcarbazepine, phenytoin and lamotrigine, a corresponding kit and a detection method of the kit. According to the HLA-B*1502 genotyping, three groups of MIX reaction solutions are detected by adopting a PCR-fluorescent probe method, and target nucleic acid molecules are circularly amplified through an amplification reaction, so that a fluorescence generation group is indirectly combined with an amplified target nucleotide sequence; the amount of fluorescence generated by the fluorescence generation group is determined, and the existence of the target nucleotide is determined. The detection reagent comprises a nucleic acid amplification system of three groups of MIX reaction solutions, wherein the nucleic acid amplification system comprises an upstream primer 1 and a downstream primer 1 which can be combined with target nucleotide, and an upstream primer 2 and a downstream primer 2 which can be combined with the target nucleotide; and three groups of probes 1 and probes 4 of a fluorescence detection system matched with the nucleic acid amplification system.
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Description

Technical field

[0001] The present invention belongs to the field of biotechnology, and more particularly to human white blood cell antigen B sites for human leuvisulphic antigenic drug medication guidance in Kamasipine, Okasi, phenytoin, Ramamazine (HLA-B * 1502) Detection reagents, detection methods and kits. The present invention also includes the use of the above-described detecting reagent in the preparation of the kits for epileptic patients to take cardaasi, Okazi, phenytoin, mamitastic treatment, and the corresponding kit and the use of the same. Background technique

[0002] Mental illness has become a global high-haired disease. According to statistics, more than 17% of my country's adults were deeply plagued by mental illness such as schizophrenia and epilepsy (Phillips.2009). Epilepsy is defined by the World Health Organization (WHO) and the International Anti-epileptic Union (ILA) as: a chronic brain disease caused by multiple causes, mainly due to the repeated and e...

Claims

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