Application of heat-clearing and stasis-removing tablet in preparation of drugs for preventing and treating leukemia

A technology for clearing away heat and dispelling stagnation and leukemia, applied in the field of medicine

Inactive Publication Date: 2021-04-06
THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, with the increase of clinical drugs, the drug resistance of related leukemi

Method used

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  • Application of heat-clearing and stasis-removing tablet in preparation of drugs for preventing and treating leukemia
  • Application of heat-clearing and stasis-removing tablet in preparation of drugs for preventing and treating leukemia
  • Application of heat-clearing and stasis-removing tablet in preparation of drugs for preventing and treating leukemia

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Experimental program
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Embodiment 1

[0018] Application of Qingre Sanjie Tablets in Preparation of Drugs for Prevention and Treatment of Leukemia

[0019] 1. Activity test of anti-leukemia cells in vitro

[0020] (1) Experimental materials

[0021] Preparation of sample solution: Qingre Sanjie Tablets were prepared into a sample solution with a concentration of 200 mg / mL with physiological saline, filtered through a 0.45 μm sterile filter, and stored at -20°C for later use.

[0022] Preparation of 5% MTT solution: Weigh 0.5 g of MTT powder, add 100 mL of sterilized PBS buffer solution, heat to dissolve at 60°C, filter and sterilize with a 0.22 μm sterile filter, and store in the dark at 4°C.

[0023] Cell lines: Leukemic cells Jurkat, K562, HEL, Daudi, and CB3 were preserved by the Key Laboratory of Natural Product Chemistry, Chinese Academy of Sciences, Guizhou Province, and human normal liver cells 7702 were preserved by the Key Laboratory of Natural Product Chemistry, Chinese Academy of Sciences, Guizhou Prov...

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Abstract

The invention discloses application of a heat-clearing and stasis-removing tablet in preparation of a drug for preventing and treating leukemia. Different human leukemia cell strains HEL, K562, Daudi and Jurkat and a mouse leukemia cell strain CB3 are applied in vitro for detection, it is found that the heat-clearing and stasis-removing tablet has a remarkable effect of inhibiting leukemia cell proliferation. The median inhibitory concentration IC50 values of the strains in 72h are as follows: CB3: 0.134 +/-0.012 mg/mL; Jurkat: 0.792+/-0.296mg/mL; Daudi: 0.775+/-0.052mg/mL; HEL: 1.027+/-0.625mg/mL; K562: 1.756+/-0.406mg/mL; and 7702: 7.153+/-1.073mg/mL. Meanwhile, a plurality of signal paths such as PI3K-AKT, MAPK, mTOR and NF-kappa B can be blocked to induce apoptosis of human acute lymphocytic leukemia cells Jurkat; and the heat-clearing and stasis-removing tablet can be used as a preparation for preventing and treating leukemia to be prepared into pharmaceutically allowable oral liquid, injection, tablets, capsules, dripping pills and the like.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of a Qingre Sanjie tablet in the preparation of medicines for preventing and treating leukemia. Background technique [0002] Leukemia is a heterogeneous blood tumor. Clonal leukemia cells proliferate and accumulate in bone marrow and other hematopoietic tissues due to mechanisms such as uncontrolled proliferation, differentiation disorder, and apoptosis inhibition, and spread to various tissues and organs throughout the body, resulting in normal hematopoietic function. Inhibited, resulting in immune collapse. Environmental pollution and other factors lead to a rapid increase in the number of newly diagnosed leukemia patients, especially childhood leukemia. At present, the incidence of leukemia ranks sixth in the incidence of tumors, and ranks first in the incidence and mortality of malignant tumors in children and adolescents. Leukemia has a high d...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61K9/20A61P35/02
CPCA61K9/20A61K36/28A61P35/02
Inventor 饶青李艳梅吴小森李静
Owner THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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