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Application of Miao medicine Liaoshi Huafengdan in the preparation of drugs for preventing and treating melanoma

A technology of melanoma and Huafeng Dan, which is applied in the fields of biology and medicine, can solve the problems of unfavorable treatment effect, insensitivity to radiotherapy and chemotherapy of melanoma, achieve good in vitro inhibitory effect, increase market share, and be well applied foreground effect

Active Publication Date: 2021-07-16
THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current treatment methods for malignant melanoma include chemotherapy, targeted therapy, immunotherapy and radiotherapy, etc., but melanoma is not sensitive to radiotherapy and chemotherapy, so the treatment effect is not optimistic. Most of the biological immunotherapy, such as melanoma vaccine, is still under development or clinical trial stage

Method used

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  • Application of Miao medicine Liaoshi Huafengdan in the preparation of drugs for preventing and treating melanoma
  • Application of Miao medicine Liaoshi Huafengdan in the preparation of drugs for preventing and treating melanoma
  • Application of Miao medicine Liaoshi Huafengdan in the preparation of drugs for preventing and treating melanoma

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Anti-melanoma cell activity test of Miao medicine Liaoshi Huafengdan in vitro

[0015] 1. Experimental materials

[0016] Preparation of sample solution: Liao's Huafeng Dan was prepared with DMSO into sample solutions with concentrations of 50 μg / mL, 25 μg / mL, 12.5 μg / mL, 6.25 μg / mL, and 3.125 μg / mL.

[0017] Preparation of 5% MTT solution: Weigh 0.5g of MTT powder, add 100mL of sterilized PBS, dissolve at 60°C, sterilize with a 0.22μm filter membrane, and store at 4°C.

[0018] Cell lines: Human melanoma cells WM35 and WM239 were preserved in the Key Laboratory of Natural Product Chemistry, Chinese Academy of Sciences, Guizhou Province. Human melanoma cells WM35, WM239 at 37°C 5% CO 2 Cultured in DMEM medium containing 5% FBS in an incubator.

[0019] DMEM medium and trypsin were purchased from Hycone Company, fetal bovine serum (FBS) was purchased from Shanghai Jiyuan Biotechnology Co., Ltd., tetramethylazobium salt (MTT) and dimethylsulfoxide (DMSO) were purchased...

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Abstract

The invention discloses the application of Miao medicine Liao's Huafengdan in the preparation of melanoma prevention and treatment drugs. It is found through the detection of melanoma cells WM35 and WM239 in vitro that Liaoshi's Huafengdan has a significant effect of inhibiting melanoma, and through the flow The results showed that Liao's Huafengdan had a significant effect on the cell cycle of melanoma, and the higher the drug concentration, the more obvious its blockage. It can be used as a preparation for preventing and treating melanoma. Permitted oral liquid, injection, tablet, capsule, drop pill, etc.

Description

technical field [0001] The invention belongs to the technical fields of biology and medicine, and in particular relates to the application of Miao medicine Liaoshi Huafengdan in the preparation of melanoma medicine. Background technique [0002] Miao medicine Liao's Huafeng Pill, manufactured by Guizhou Wansheng Pharmaceutical Co., Ltd., is a pill. Its existing functions are: relieving wind and spasm, eliminating phlegm and resuscitation. For wind-phlegm obstruction, stroke hemiplegia, epilepsy, facial paralysis, crooked mouth and eyes. The main ingredients include Gastrodia elata, scorpion, silkworm, aconite, atractylodes, araceae, musk and other 20 aftertaste Chinese herbal medicines. Vermilion water pills, the profile is brownish yellow; it has a strong aroma and acrid taste. [0003] Melanoma is a tumor that arises from melanocytes in the skin or other organs and is the most aggressive skin tumor. Although the incidence of melanoma in my country accounts for a small p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8988A61P35/00A61K35/646A61K35/64A61K35/55A61K33/36A61K33/28A61K33/22A61K31/045
CPCA61K31/045A61K33/22A61K33/28A61K33/36A61K35/55A61K35/64A61K35/646A61K36/185A61K36/284A61K36/47A61K36/535A61K36/538A61K36/8884A61K36/8988A61P35/00A61K2300/00
Inventor 宋晶睿李艳梅陈娟饶青
Owner THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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