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A Polymorphic Microsatellite Molecular Marker, Primer and Application

A technology of molecular markers and microsatellites, applied in the field of DNA molecular markers in molecular biology, can solve problems such as vulnerability, resource reduction, and deterioration of living environment, and achieve high polymorphism, stable markers, and good repeatability

Active Publication Date: 2022-07-01
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, its living environment has deteriorated, and its resources have decreased sharply. It is now in a vulnerable state, and it is urgent to vigorously carry out the protection of aquatic germplasm resources.

Method used

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  • A Polymorphic Microsatellite Molecular Marker, Primer and Application
  • A Polymorphic Microsatellite Molecular Marker, Primer and Application
  • A Polymorphic Microsatellite Molecular Marker, Primer and Application

Examples

Experimental program
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Embodiment 1

[0105] 1. Transcriptome Sequencing

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Abstract

The invention belongs to the technical field of molecular biology DNA molecular markers, and discloses a polymorphic microsatellite molecular marker, primers and applications of the polymorphic microsatellite molecular marker of the balsamina chinensis. / R, 57324F / R, 29968F / R, 6321F / R, 17156F / R, 16775F / R, 23957F / R, 11365F / R, 50660F / R, 2904F / R, 45390F / R, 43219F / R, 20549F / R , 9153F / R, 31370F / R and 852F / R, the nucleotide sequences of the polymorphic microsatellite molecular markers of the fish lacking beetles are shown in SEQ.ID.No.1-12 respectively. The present invention can be used for the analysis of population genetic diversity and population genetic structure of A. chinensis.

Description

technical field [0001] The invention belongs to the technical field of molecular biology DNA molecular markers, and in particular relates to a polymorphic microsatellite molecular marker, primers and applications of A. Background technique [0002] At present, Placocheilus cryptonemus, also known as oil fish, belongs to Cyprinidae (Cyprinidae), Labeoninae (Labeoninae), and Placocheilus (Placocheilus). , the body is nearly cylindrical. Pseudomonas chinensis is an endemic species of the Nu River. In recent years, the name of Pseudomonas serrata has often appeared in discussions on the protection of fish resources in the Nujiang River Basin, but there are few studies on it, only limited to its biology and morphology. few studies. In 2004, the "Red List of Chinese Species" has been classified as vulnerable, and better protection of the fish is a problem that needs to be studied and explored urgently. [0003] Microsatellite markers (microsatellite), also known as short tandem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
Inventor 马秀慧任芳黄福江
Owner GUIZHOU UNIV
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