Parasite rapid enzyme immunoassay method and kit for salmon and trout culture

A technology for detecting kits and parasites, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of poor specificity, high cost, low efficiency, etc., and achieve the effect of simple preparation method and accurate identification

Pending Publication Date: 2021-04-20
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of poor specificity, low efficiency and high cost of existing parasite detection methods in the field of salmon and trout, the present invention provides a rapid enzyme immunological detection method and kit for parasites used in salmon and trout farming

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This embodiment provides a rapid enzyme immunoassay kit for third-generation salmon and trout cultured worms.

[0028] The detection kit of this embodiment includes: third-generation worm-specific antigen-coated plate, 0.1M phosphate buffer containing 0.5% Tween, enzyme-labeled antigen solution, 30% hydrogen peroxide solution, 2mg / mL tetramethylbenzidine Absolute ethanol solution, 2N sulfuric acid solution, positive control serum and negative control serum.

[0029] In this embodiment, the preparation steps of the three-generation worm-specific antigen-coated plate are as follows:

[0030] Step 1. Prepare soluble antigens of third-generation worms: remove the third-generation worms parasitic on fish under a microscope, and put them in normal saline. When the density of worms reaches 10 6 ~10 7 / mL, centrifuge at 4°C to collect the worms, wash the worms three times with PBS buffer solution of pH 7.0, add 5 times the volume of PBS buffer solution of pH 7.0 to the washed...

Embodiment 2

[0035] This embodiment provides a rapid enzyme immunoassay kit for the cultured salmon and trout.

[0036] The detection kit of this example includes: small melon worm-specific antigen-coated plates, 0.1M phosphate buffer containing 0.5% Tween, enzyme-labeled antigen solution, 30% hydrogen peroxide solution, 2 mg / mL tetramethyl-linked Anhydrous ethanol solution of aniline, 2N sulfuric acid solution, positive control serum and negative control serum.

[0037] In this embodiment, the preparation steps of the small melon worm-specific antigen-coated plate are as follows:

[0038]Step 1. Prepare the soluble antigen of the small melon worms: remove the small melon worms parasitic on the fish under a microscope and put them into physiological saline, and the density of the worms reaches 10 6 ~10 7 / mL, centrifuge at 4°C to collect the worms, wash the worms three times with PBS buffer solution of pH 7.0, add 5 times the volume of PBS buffer solution of pH 7.0 to the washed worms ac...

Embodiment 3

[0043] This embodiment provides a rapid enzyme immunoassay kit for trichomonas in salmon and trout culture.

[0044] The detection kit of this embodiment includes: trichotillotype-specific antigen-coated plate, 0.1M phosphate buffer containing 0.5% Tween, enzyme-labeled antigen solution, 30% hydrogen peroxide solution, 2mg / mL tetramethylbenzidine Absolute ethanol solution, 2N sulfuric acid solution, positive control serum and negative control serum.

[0045] In this embodiment, the preparation steps of the trichotillotype-specific antigen-coated plate are as follows:

[0046] Step 1. Preparation of soluble antigens of Trichomonas worms: Remove the Trichomonas parasitic on the fish body under a microscope and put them in normal saline until the density of the worms reaches 10 6 ~10 7 / mL, centrifuge at 4°C to collect the worms, wash the worms three times with PBS buffer solution of pH 7.0, add 5 times the volume of PBS buffer solution of pH 7.0 to the washed worms according t...

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Abstract

The invention relates to a parasite rapid enzyme immunoassay method and kit for salmon and trout culture, and belongs to the technical field of cultured fish parasite detection. In order to solve the problems of poor specificity, low efficiency and high cost of an existing parasite detection method in the salmon and trout field, the invention provides the parasite rapid enzyme immunoassay kit for salmon and trout culture. The kit comprises a parasite specific antigen coated plate, a 0.1 M phosphate buffer solution containing 0.5% Tween, an enzyme-labeled antigen solution, a 30% hydrogen peroxide solution, a tetramethyl benzidine color developing agent, a 2N sulfuric acid solution, positive control serum and negative control serum. The detection method and the kit provided by the invention have good specificity and sensitivity for various common parasites in salmon and trout culture, and can be used for rapidly, efficiently and accurately identifying the species of parasites infected by fish bodies and accurately guiding medication. The detection kit disclosed by the invention is low in cost, simple to operate and suitable for field use of large-scale salmon and trout farms.

Description

technical field [0001] The invention belongs to the technical field of parasite detection in cultured fish, and in particular relates to a rapid enzyme immunological detection method and kit for parasites used in salmon and trout farming. Background technique [0002] Salmon trout is a typical cold-water fish with high economic value and is one of the most important economically farmed fish in the world. However, with the increasing production of salmon and trout, the occurrence and damage of salmon and trout parasitic diseases caused by the increase of breeding density and the deterioration of the breeding environment are also becoming more frequent and serious, which restricts the healthy development of the salmon and trout farming industry. The more serious parasites common to salmon and trout mainly include third-generation worms, small melon worms, and trichotillomantes. These parasites have a simple life history and fast reproduction speed, and most of them parasitize...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/543G01N33/535G01N33/531
Inventor 卢彤岩韩世成刘红柏邵轶智陈福广
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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