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HLA-G specific chimeric antigen receptor, nucleic acid encoding HLA-G specific chimeric antigen receptor, expression plastid of HLA-G specific chimeric antigen receptor, cell expressing HLA-G specific chimeric antigen receptor, application of cell and composition

A chimeric antigen receptor, HLA-G technology, applied in nucleic acid encoding chimeric antigen receptors, pharmaceutical compositions for treating cancer, chimeric antigen receptors, and cells expressing chimeric antigen receptors, can solve intractable problems Overcoming solid tumor immunosuppressive microenvironment, inefficient immune cells, and chimeric antigen receptor efficacy limitations

Pending Publication Date: 2021-04-27
CHINA MEDICAL UNIV HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of unique tumor-associated antigens, the low efficiency of homing immune cells to tumor sites, and the inability to overcome the immunosuppressive microenvironment of solid tumors, the current cell therapy of chimeric antigen receptor immune cells makes chimeric antigen receptor Very limited efficacy in solid tumors

Method used

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  • HLA-G specific chimeric antigen receptor, nucleic acid encoding HLA-G specific chimeric antigen receptor, expression plastid of HLA-G specific chimeric antigen receptor, cell expressing HLA-G specific chimeric antigen receptor, application of cell and composition
  • HLA-G specific chimeric antigen receptor, nucleic acid encoding HLA-G specific chimeric antigen receptor, expression plastid of HLA-G specific chimeric antigen receptor, cell expressing HLA-G specific chimeric antigen receptor, application of cell and composition
  • HLA-G specific chimeric antigen receptor, nucleic acid encoding HLA-G specific chimeric antigen receptor, expression plastid of HLA-G specific chimeric antigen receptor, cell expressing HLA-G specific chimeric antigen receptor, application of cell and composition

Examples

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Effect test

Embodiment 1

[0039] In this test example, the HLA-G-specific chimeric antigen receptor of the present invention is transduced into primary natural killer cells to obtain the HLA-G-specific chimeric antigen receptor-expressing cells of Example 1 of the present invention (hereinafter It is referred to as Example 1) for short. And the effect of Example 1 and the pharmaceutical composition for treating cancer containing Example 1 in inducing the death of breast cancer cells, glioblastoma multiforme cells, pancreatic cancer cells and ovarian cancer cells was further tested.

[0040] Firstly, human breast cancer cell line MDA-MB-231, human malignant brain tumor cell line DBTRG, human pancreatic cancer cell line AsPC1 and human ovarian cancer cell line SKOV3 were mixed with 1×10 5 The density of cells / well was seeded in 12-well plates, and the experiment was performed after culturing for 48 hours. In the experiment, each tumor cell was divided into 6 groups, which were untreated control group, e...

Embodiment 2

[0048] In this test example, the HLA-G-specific chimeric antigen receptor expressing cell of the present invention Example 2 (hereinafter referred to as For example 2). And the effect of Example 2 and the pharmaceutical composition for treating cancer comprising Example 2 in inducing the death of breast cancer cells, glioblastoma multiforme cells, pancreatic cancer cells and ovarian cancer cells was further tested.

[0049] Firstly, human breast cancer cell line MDA-MB-231, human malignant brain tumor cell line DBTRG, human pancreatic cancer cell line AsPC1 and human ovarian cancer cell line SKOV3 were mixed with 1×10 5 The density of cells / well was seeded in 12-well plates, and the experiment was performed after culturing for 48 hours. In the experiment, each tumor cell was divided into 6 groups, namely the untreated control group, the experimental group 1 treated with chemotherapy drugs, the experimental group 2 treated with parental primary T cells, and the experimental gr...

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Abstract

The invention discloses an HLA-G specific chimeric antigen receptor, nucleic acid encoding the HLA-G specific chimeric antigen receptor, an expression plastid of the HLA-G specific chimeric antigen receptor, a cell expressing the HLA-G specific chimeric antigen receptor, an application of the cell and a composition, The present invention relates to the HLA-G specific chimeric antigen receptor, an isolated nucleic acid, the expression plastid of the HLA-G specific chimeric antigen receptor, the cell expressing the HLA-G specific chimeric antigen receptor, a pharmaceutical composition for treatment of cancer, and the application of the cell expressing the HLA-G specific chimeric antigen receptor. The HLA-G specific chimeric antigen receptor and a human leukocyte antigen G are specifically bound. The cell expressing the HLA-G specific chimeric antigen receptor is obtained by transducing the HLA-G specific chimeric antigen receptor into an immune cell. The pharmaceutical composition for treatment of cancer comprises the cell expressing the HLA-G specific chimeric antigen receptor and a pharmaceutically acceptable carrier. Therefore, the cell expressing the HLA-G specific chimeric antigen receptor can be used for inducing tumor cell death of mammals.

Description

technical field [0001] The present invention relates to a pharmaceutical product containing antigen or antibody, especially a chimeric antigen receptor, nucleic acid encoding chimeric antigen receptor, chimeric antigen receptor expressing plastid, and chimeric antigen receptor expressing cell , a pharmaceutical composition for treating cancer and uses of cells expressing chimeric antigen receptors. Background technique [0002] Cancer, also known as malignant tumor, is an abnormal proliferation of cells, and these proliferating cells may invade other parts of the body. It is a disease caused by abnormal control of cell division and proliferation mechanisms. The number of people suffering from cancer is increasing all over the world. Cancer is one of the top ten causes of death in China, and it has been the top ten cause of death for 27 consecutive years. [0003] Conventional tumor treatment methods include surgery, radiation therapy, chemotherapy, and targeted therapy. Tu...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62C12N15/867C12N5/10A61K39/00A61P35/00
CPCC07K16/2833C07K14/7051C07K14/7155C07K14/70517C12N15/86C12N5/0636C12N5/0646A61K39/001111A61K39/001119A61P35/00C07K2317/622C07K2317/565C07K2319/02C07K2319/03C07K2319/33C12N2740/15043C12N2800/107C12N2510/00A61K2039/5156A61K2039/812A61K2039/80A61K2039/852A61K2039/892
Inventor 周德阳邱绍智詹佳颖潘志明黄士维
Owner CHINA MEDICAL UNIV HOSPITAL
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