Gene sequencing measuring device and measuring method thereof

A measurement device and gene sequencing technology, applied in the direction of measurement devices, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems that it is difficult to avoid the nucleotide label blocking current being read, missed reading, etc., to avoid Omissions and over-reading errors, the effect of improving accuracy

CN112708544AInactive Publication Date: 2021-04-27GENEUS TECH CHENGDU CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2021-04-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The embodiment of the invention provides a gene sequencing measuring device and a measuring method thereof. The measuring device comprises at least one detection unit, wherein the detection unit comprises a test cavity and a measuring circuit unit connected to the test cavity, the measuring circuit unit comprises a first amplifying circuit and a second amplifying circuit and is used for modulating and amplifying a current signal caused by bidirectional movement of nucleotide molecules between a first compartment and a second compartment through nanopores in a membrane, and outputting a first characteristic voltage signal and a second characteristic voltage signal to judge the types of the nucleotide molecules and whether to participate in synthesis reaction and further complete sequencing.
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Description

technical field

[0001] The disclosure belongs to the technical field of biological detection, and in particular relates to a measuring device for gene sequencing and a measuring method thereof, which can be used to detect the base type of nucleotides to realize sequencing. Background technique

[0002] The concept of nucleic acid sequencing based on nanopores was proposed in 1995. Researchers have found that certain transmembrane proteins, such as the bacterial toxin α-hemolysin, can form stable channels with a diameter of about 1-2 nanometers on phospholipid membranes, called nanopores (nanopore). Single-stranded DNA (or RNA) molecules will spontaneously pass through nanopores in an electric field due to their own charged properties, and cause changes in the resistance of nanopores during the passage, resulting in a so-called blocking current. Due to the differences in their own chemical structures, the four different bases of DNA (RNA), A, T (U), C, and G, have identifiab...

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Embodiment Construction

[0039] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings in the embodiments of the present disclosure. Apparently, the described embodiments are some of the embodiments of the present disclosure, not all of them. Based on the embodiments in the present disclosure, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0040] In the present disclosure, it should be understood that terms such as "comprising" or "having" are intended to indicate the presence of features, numbers, steps, acts, components, parts or combinations thereof disclosed in the specification, and are not intended to exclude one or a plurality of other features, numbers, steps, acts, parts, parts ...