Gene sequencing measuring device and measuring method thereof

Abstract

The embodiment of the invention provides a gene sequencing measuring device and a measuring method thereof. The measuring device comprises at least one detection unit, wherein the detection unit comprises a test cavity and a measuring circuit unit connected to the test cavity, the measuring circuit unit comprises a first amplifying circuit and a second amplifying circuit and is used for modulating and amplifying a current signal caused by bidirectional movement of nucleotide molecules between a first compartment and a second compartment through nanopores in a membrane, and outputting a first characteristic voltage signal and a second characteristic voltage signal to judge the types of the nucleotide molecules and whether to participate in synthesis reaction and further complete sequencing.

Description

technical field [0001] The disclosure belongs to the technical field of biological detection, and in particular relates to a measuring device for gene sequencing and a measuring method thereof, which can be used to detect the base type of nucleotides to realize sequencing. Background technique [0002] The concept of nucleic acid sequencing based on nanopores was proposed in 1995. Researchers have found that certain transmembrane proteins, such as the bacterial toxin α-hemolysin, can form stable channels with a diameter of about 1-2 nanometers on phospholipid membranes, called nanopores (nanopore). Single-stranded DNA (or RNA) molecules will spontaneously pass through nanopores in an electric field due to their own charged properties, and cause changes in the resistance of nanopores during the passage, resulting in a so-called blocking current. Due to the differences in their own chemical structures, the four different bases of DNA (RNA), A, T (U), C, and G, have identifiab...

AI Technical Summary

Problems solved by technology

Although tagging the nucleotides used for replication can improve the recognition of the characteristic blocking currents corresponding to different bases, at the same time, the time interval between the entry of a single nucleotide tag into the nanopore is also helpful for the detection of consecutive nucleic acids with the same base sequence (homopolymer), but this system is difficult to ensure that the label of each nucleotide used for synthesis enters the nanopore to give a blocking current, resulting in a deletion error during the sequencing process; it is also difficult to avoid nucleoside The acid tag blocks the current from being read, but the nucleotide itself does not actually participate in the synthesis reaction, resulting in an error in which the signal is read redundantly (insertion error)

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