A method for preparing sodium aescinate by extracting sorghum seeds

A technology of sodium aescinate and aescin, applied in the field of natural medicinal chemistry, achieving the effects of environmental friendliness, simple process conditions, and good wall-breaking effect

Active Publication Date: 2021-11-30
SHANGHAI JAKA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the use of micellar technology in the purification process of aescin

Method used

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  • A method for preparing sodium aescinate by extracting sorghum seeds
  • A method for preparing sodium aescinate by extracting sorghum seeds

Examples

Experimental program
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Effect test

Embodiment 1

[0038] The present embodiment provides a method for preparing Sodium Aescinate by extracting from the seeds of Sala, the preparation method comprising the following steps:

[0039] (1) Grinding 100 g of sala seed raw material (a horse chestnut seed from the place of production in Hubei) to a particle size of 8 to 20 mesh.

[0040] (2) Add 300-400g of acidic water with a pH value of 6.0-6.9 to the crushed sala seed raw material, mix evenly, spread it on a plastic tray and send it to the freezer. After freezing, use microwave to thaw, thaw The temperature is not higher than 40°C to achieve wall breaking.

[0041] (3) Add 800 to 1000 g of acidic ethanol aqueous solution (ethanol concentration is 30% to 50%, pH value is 5.0 to 6.9) to the broken Sala seed raw material, and extract for 3 hours at a temperature of 40 to 45°C Above, obtain the initial extraction liquid.

[0042] (4) After the initial extract is concentrated to less than 1 / 4 of the original volume, a concentrated so...

experiment example 1

[0052] According to the detection method disclosed in the enterprise standard Q / C-H0026-4 "Aescinus extract" of Shanghai Jiakai Biotechnology Co., Ltd., the purity of the product was analyzed by high performance liquid chromatography. The sodium aescinate prepared in Example 1 and Comparative Example 1 was subjected to chromatographic detection.

[0053] Accurately weigh 5.0 mg of the aescin control reagent product with the identified aescin A content, and use methanol to make up the volume to prepare the aescin standard solution. The chromatographic conditions for sample detection are: C18 chromatographic column (4.6mm×250mm, 5 μm); the mobile phase is acetonitrile:0.3% phosphoric acid aqueous solution (volume ratio 33:67); the detection wavelength is 220nm (nanometer); the column temperature is 35°C; : l ml / min.

[0054] Take aescin standard solution and inject 1 μl (microliter), 2 μl, 4 μl, 8 μl, 10 μl respectively for chromatographic analysis. With the concentration of ea...

experiment example 2

[0060] According to the detection method disclosed in "Chinese Pharmacopoeia" (2020 Edition, Part One, Page 305, Sala Seed), high performance liquid chromatography (general rule 0512) was used to determine the content of β-aescin in the Sala Seed raw material.

[0061] Octadecylsilane bonded silica gel is used as filler; mobile phase is acetonitrile: 0.2% phosphoric acid solution (36:64); detection wavelength is 220nm (nanometer). Take aescin sodium reference substance (the contents of aescin A and aescin B have been marked) and add methanol to make a reference solution. Take about 1 gram of the salsa seed material used in Example 1, accurately weigh it, and place it in a Soxhlet extractor, add ether, heat and reflux for 1 hour, discard the ether solution, evaporate the solvent with the dregs and the filter paper tube, and place it in a Soxhlet extractor. In a conical flask with a stopper, add 50 ml of methanol precisely, weigh it, ultrasonically treat it (power 250W, frequenc...

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Abstract

The invention provides a method for preparing sodium aescinate by extracting from seeds of salsa. The preparation method comprises the following steps: crushing the raw material of the sala seeds and breaking the walls; leaching the crushed and broken walls of the sala seed raw materials with an aqueous acidic ethanol solution to obtain an initial extraction liquid; after concentrating the initial extraction liquid, eluting the grease with a solvent to obtain an aqueous phase solution; Adding a polymer surfactant to the aqueous phase solution and performing rotary evaporation to obtain a escin concentrate; decolorizing the aescin concentrate; performing sodium-type cation exchange resin chromatography on the decolorized aescin concentrate, collecting The eluate is obtained; the eluate is filtered, crystallized, recrystallized and then dried to obtain sodium aescinate. The process conditions of the present invention are relatively simple and environment-friendly; the high-molecular polymer surfactant it adopts can effectively remove impurities, can selectively form micelles with β-aescin, and the purity of the prepared sodium aescinate is greater than 95 %, wherein the mass content of β-aescin sodium is as high as more than 70%.

Description

technical field [0001] The invention belongs to the technical field of natural medicine chemistry, and relates to a method for preparing sodium aescinate by extracting and extracting from seeds of aescin. Background technique [0002] Aesculi (Aesculi, also known as Su Luozi, Ling Luozi) is the horse chestnut (Aesculuschinensis Bge), European horse chestnut (Aesculus hippocastanum), Tianshi chestnut (Aesculus wilsoniiRehd) or Zhejiang horse chestnut (Aesculus wilsoniiRehd) Mature seeds of Aesculus chinensis Bge.var.chekiangensis Fang). As a commonly used traditional Chinese medicine, Su Luozi is sweet and warm in nature and taste, and returns to the liver and stomach meridians. It has the effects of regulating qi, relaxing the middle, harmonizing the stomach and relieving pain. Sala seeds mainly contain 32% of fatty oil, 36% of starch, 15% of fiber, etc., wherein the fatty oil is mainly glyceride of oleic acid and stearic acid. Aescin is widely distributed in my country, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08
CPCC07H1/08C07H15/256C07J63/008
Inventor 田军王新亮刘青卢伊娜
Owner SHANGHAI JAKA BIOTECH CO LTD
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