Method for increasing content of alkaloid in lycoris radiata through exogenous substance

A technology of alkaloids and exogenous substances, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve problems such as shortage

Inactive Publication Date: 2021-05-07
SHANGHAI ELITE AGRI SCI TECH GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of research on the growth and alkaloid accumulation of Lycoris longifolia by precursors and elicitors. Therefore, it is necessary

Method used

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  • Method for increasing content of alkaloid in lycoris radiata through exogenous substance
  • Method for increasing content of alkaloid in lycoris radiata through exogenous substance
  • Method for increasing content of alkaloid in lycoris radiata through exogenous substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]Select 3-month-old tissue-cultured seedlings of Lycoris in uniform growth state, inoculate them on the liquid medium of MS+6-BA 3.0mg / L+NAA 1mg / L+30g / L sucrose, and fill each bottle with 20ml of medium. Add 0.22 μm filter-sterilized 4'-O-methylnoramaryllin (MN) solution to the medium, and set the final concentrations to 0.1 g / L MN (T1) and 0.2 g / L MN (T2), respectively. Each bottle of liquid medium was inoculated with 2g of Amaryllis lycoris tissue culture seedlings, and each liquid medium with different treatments needed to inoculate 3 bottles, with 3 replicates, at 23°C, 50μmol / m 2 / s light intensity for 12 hours, the oscillation frequency is 100r / min. They were taken out after 7d, 14d and 30d of culture in liquid medium.

Embodiment 2

[0040] Select Lycoris annual tissue culture seedlings with uniform growth state and inoculate them on the liquid medium of MS+6-BA 3.0mg / L+NAA 1mg / L+30g / L sucrose, and fill each bottle with 20ml of medium. Add 0.22 μm filter-sterilized 4'-O-methylnoramaryllin (MN) solution to the medium, and set up 3 treatments so that the final concentrations are 0g / L (T0), 0.1g / L MN (T1), 0.2g / L MN(T2), each bottle of liquid medium was inoculated with 2g long-tube Lycoris tissue culture seedlings, each liquid medium with different treatments needed to inoculate 3 bottles, set 3 replicates, at 25°C, 50μmol / m 2 Under the light intensity of / s light for 12 hours, the oscillation frequency is 110r / min. After being cultured in liquid medium for 7d, 14d and 30d, they were taken out, and the contents of three alkaloids, galantamine, ricolamine and lycorine, were determined by a chromatograph connected in series with a secondary mass spectrometer.

[0041] The measurement results are shown in Tab...

Embodiment 3

[0049] Select 1-year-old tissue culture seedlings of Amaryllis lycoris with uniform growth state, inoculate them on the liquid medium of MS+6-BA 3.0mg / L+NAA 1mg / L+30g / L sucrose, and fill each bottle with 20ml of medium. Add 0.22 μm filter-sterilized methyl jasmonate (MeJA) solution to the medium, and set the final concentrations as 75 μmol / L MeJA (MJ-75), 150 μmol / L MeJA (MJ-150) and 300 μmol / L MeJA (MJ -300), each bottle of liquid medium was inoculated with 2g of Amaryllis lycoris tissue culture seedlings, each liquid medium of different treatment needs to inoculate 3 bottles, set 3 repetitions, at 27°C, 50μmol / m 2 / s light intensity for 12 hours, the oscillation frequency is 120r / min. After being cultured in liquid medium for 7d, 14d and 30d, they were taken out, and the contents of three alkaloids, galantamine, ricolamine and lycorine, were determined by a chromatograph connected in series with a secondary mass spectrometer.

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Abstract

The invention provides a method for increasing the content of alkaloid in lycoris radiata through an exogenous substance, and relates to the field of plant growth and secondary metabolite accumulation regulation. According to the method for increasing the content of the alkaloid in the lycoris radiata through the exogenous substance, 3-month-to-one-year-old tissue culture seedlings of the lycoris radiata are treated through the exogenous substance 4 '-O-methyl norlinaldine and/or jasmonic acid methyl ester, so that the content of three alkaloids, that is, galanthamine, lycorine and lycorine which have important medicinal values in the lycoris radiata are effectively increased.

Description

technical field [0001] The invention relates to the technical field of regulation and control of plant growth and secondary metabolite accumulation, in particular to a method for increasing the alkaloid content in Lycoris by using exogenous substances. Background technique [0002] Lycoris longituba (Lycoris longituba) is a bulbous herb of the family Amaryllidaceae. It has high ornamental value and also has medicinal functions. It is rich in a variety of alkaloid components unique to Amaryllidaceae plants. Lycorine (LYC) can induce cell apoptosis in the cancer cell line HL-60, and galantamine (GAL) and ricolamine (LYCM) are one of the first-choice drugs for the clinical treatment of Alzheimer's disease. However, the content of the above three alkaloids in wild Amaryllis plants is relatively low (only a few parts per million to a few parts per million), and is also affected by provenance, developmental period and environmental conditions, and the yield is low, which is far fr...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01H4/00
CPCA01G7/06A01H4/001A01H4/008
Inventor 李青竹蔡友铭张永春许俊旭
Owner SHANGHAI ELITE AGRI SCI TECH GROUP
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