Hematopoietic stem cell cryopreservation solution and cryopreservation method
A technology of hematopoietic stem cells and cryopreservation methods, applied in the preservation, application, and animal husbandry of human or animal bodies, which can solve the problems of poor safety and achieve the effects of maintaining stability, avoiding water crystallization, and changing permeability
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Embodiment 1
[0022] A cryopreservation solution for hematopoietic stem cells, including basal medium RPMI-1640 and additives, the ingredients of the additives in terms of final concentration include: rice protein 10 μg / mL, sulbutiamine 5 μg / mL, coumarin 8 μg / mL, carbamic acid Ethyl ester 12μg / mL, mannitol 25μg / mL, vitamin C 12ng / mL, naringin 15ng / mL.
[0023] A cryopreservation method for hematopoietic stem cells, comprising the following steps:
[0024] (1) Take the basal medium RPMI-1640 and add rice protein, sulbutiamine, coumarin, ethyl carbamate, mannitol, vitamins, and naringin, and stir evenly at 45°C to obtain a cryopreservation solution;
[0025] (2) Add hematopoietic stem cells to be preserved into the cryopreservation solution of step (1), and the concentration of hematopoietic stem cells in the cryopreservation solution is 3×10 7 pcs / mL, divided into 2mL cryovials, each containing 1.5mL, cooled the cryovials at a rate of 1°C / min, cooled to -20°C, and then put them into -80°C f...
Embodiment 2
[0027] A hematopoietic stem cell cryopreservation solution, including basal medium RPMI-1640 and additives, the ingredients of which are calculated by final concentration: rice protein 8 μg / mL, sulbutiamine 2 μg / mL, coumarin 5 μg / mL, carbamic acid Ethyl ester 10 μg / mL, mannitol 20 μg / mL, vitamin C 10 ng / mL, naringin 11 ng / mL.
[0028] A cryopreservation method for hematopoietic stem cells, comprising the following steps:
[0029] (1) Take the basal medium RPMI-1640 and add rice protein, sulbutiamine, coumarin, ethyl carbamate, mannitol, vitamins, and naringin, and stir evenly at 40°C to obtain a cryopreservation solution;
[0030] (2) Add hematopoietic stem cells to be preserved into the cryopreservation solution of step (1), the concentration of hematopoietic stem cells in the cryopreservation solution is 1×10 7 1 / mL, put into 2mL cryovials, each tube contains 1.5mL, cool the cryovials at a rate of 2°C / min, cool down to -25°C, and put them into -80°C for freezing.
Embodiment 3
[0032] A hematopoietic stem cell cryopreservation solution, including basal medium RPMI-1640 and additives. The ingredients of the additives include: rice protein 12 μg / mL, sulbutiamine 8 μg / mL, coumarin 10 μg / mL, carbamic acid Ethyl ester 15μg / mL, mannitol 30μg / mL, vitamin C 15ng / mL, naringin 18ng / mL.
[0033] A cryopreservation method for hematopoietic stem cells, comprising the following steps:
[0034] (1) Take the basal medium RPMI-1640 and add rice protein, sulbutiamine, coumarin, ethyl carbamate, mannitol, vitamins, and naringin, and stir evenly at 50°C to obtain a cryopreservation solution;
[0035] (2) Add hematopoietic stem cells to be preserved into the cryopreservation solution of step (1), the concentration of hematopoietic stem cells in the cryopreservation solution is 5×10 7 1 / mL, divided into 2mL cryovials, each containing 1.5mL, cooled the cryovials at a rate of 2°C / min, cooled to -30°C, and then put them into -80°C for cryopreservation.
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