Sex pheromone composition of Pinus sylvestris sylvestris and its lure and application
A technology for the sex and composition of P. sylvestris, which is applied to the P. sylvestris sex pheromone composition and its lure and application fields, can solve the problems of labor-intensive, missing coating, insignificant control effect and the like, and achieves protection Environment, pollution reduction, significant economic effect
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Embodiment 1
[0037] Example 1 Extraction and identification of the sex pheromone of Pinus sylvestris sylvestris
[0038] 1. Extraction of sex pheromones
[0039] Collect the gelatinous mass containing advanced larvae and pupae in places where the spot borer occurs seriously, put them into large net cages in the forest, and wait for the larvae to pupate and emerge under natural environmental conditions. Use virgin female moths 2-3 days after eclosion, and lightly press the end of the abdomen with your fingers 5-6 hours after entering the dark period to make the sex pheromone glands protrude, then cut off the glands, and quickly put them into the moth containing 1.0ml In a pointed test tube with n-hexane, after leaching for half an hour, the nitrogen gas was concentrated to 20-50 μl, and stored at -20°C for later use.
[0040] 2. Analysis of identifying pheromones
[0041] (1) Analysis conditions of gas chromatography-mass spectrometry: gas chromatograph Thermo ScientificTM TRACETM1300, ch...
Embodiment 2
[0044] Example 2 Production of sex pheromone lure core for Pinus sylvestris sylvestris sylvestris and moth lure test in the forest
experiment example 1
[0046] 1. Make lures (without antioxidants)
[0047] Using rubber ( Figure 5 ) as the lure slow-release carrier, and the solvent is n-hexane. Add 100 μl of the prepared sex pheromone composition solution of Pinus sylvestris sylvestris to each lure.
[0048] 2. Moth lure test in the forest
[0049] The moth trap test in the forest was carried out at the two sites of Xinjiang Experimental Forest Farm (A) and Fuyu Forest Farm (B) in Qiqihar City, Heilongjiang Province at the same time. The test date was from the beginning of July to the end of July 2020. The traps were triangular traps. Each formula of the sex pheromone composition of Pinus sylvestris sylvestris was repeated 6 times, and the traps were hung about 1.8 meters above the ground, and the distance between the traps was about 40 meters, and the traps were recorded every 3 days. moth volume. The formula of the sex pheromone composition of Pinus sylvestris sylvestris is shown in Table 1:
[0050] Table 1
[0051] ...
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