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Method for preparing licochalcone A

A technology for licorice chalcone and licorice puffs, applied in the directions of botanical equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problems of high price, low content of licorice chalcone A, slow growth of licorice, etc.

Active Publication Date: 2021-06-04
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Licochalcone A is in short supply in the market due to the slow growth of licorice, low content of licochalcone A and high price.
The biosynthetic pathway and regulatory mechanism of licochalcone A have not been reported yet.

Method used

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  • Method for preparing licochalcone A
  • Method for preparing licochalcone A
  • Method for preparing licochalcone A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Planting of Glycyrrhiza inflate seedlings

[0038] Take 200 licorice seeds, put them in a 50ml centrifuge tube, add 10ml of concentrated sulfuric acid, and soak for 15-20 minutes. Suck out the concentrated sulfuric acid, rinse with pure water 5 times, 15ml each time, until no concentrated sulfuric acid remains. Add 20ml 2% NaClO to the ultra-clean workbench and disinfect for 15-20 minutes. Then pour out the NaClO, wash with 15ml of sterile pure water 5 times, and let stand for 10min each time. After cleaning, add 20ml of sterile pure water, shake and culture at 28°C and 200rpm for 24 hours, and the seeds of Glycyrrhiza inflate begin to germinate, and newly germinated Glycyrrhiza inflate seedlings are obtained.

[0039] 2.5-azaC treatment of Glycyrrhiza inflata seedlings

[0040] Glycyrrhiza inflata seedlings grown on MS medium for 2 days were transferred to MS medium containing 10, 30, 50 μM 5-azaC and MS medium without 5-azaC for vertical culture, and Glycyrrhiza...

Embodiment 2

[0050] 1. Planting of Glycyrrhiza inflate seedlings

[0051] Method is with embodiment 1.

[0052] 2. Induction of Hairy Roots of Glycyrrhiza inflata

[0053] Dyeing licorice explants with explant dipping method: Glycyrrhiza inflata seedlings germinated on MS solid medium for 8 days, and their hypocotyls and cotyledons were cut off in a sterile ultra-clean workbench for rooting Agrobacterium infection. The dipped licorice explants were placed on 1 / 2MS medium containing 250 mg / L cephalosporin, and after the hairy roots of Glycyrrhiza swollen fruits grew 3-5 cm from the explants, the hairy roots of Glycyrrhiza swollen fruits were cut off. Roots were transferred to 1 / 2 MS medium containing 250 mg / L cephalosporin. Select the hairy roots of Glycyrrhiza inflata that can grow rapidly on the above medium and transfer them to 1 / 2MS medium containing 250mg / L cephalosporin for suspension culture. A part of the hairy roots of Glycyrrhiza inflata were taken from the above-mentioned sus...

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Abstract

The invention provides a method for preparing licochalcone A. The method comprises the following steps: culturing glycyrrhiza inflata hairy roots or glycyrrhiza inflata seedlings in a culture medium containing a DNA methyltransferase inhibitor for not less than three days, collecting roots of the glycyrrhiza inflata hairy roots or glycyrrhiza inflata seedlings, and extracting the licochalcone A. The culture medium containing the DNA methyltransferase inhibitor is used for culturing glycyrrhiza inflate seedlings or glycyrrhiza inflate hairy roots, so that the DNA methylation degree of the glycyrrhiza inflate seedlings or glycyrrhiza inflate hairy roots can be reduced; and genes which are originally silent or low in expression quantity and are beneficial to accumulation of licochalcone A are reactivated, so that the content of licochalcone A is quickly and effectively increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for preparing licochalcone A. Background technique [0002] Glycyrrhiza licorice is a perennial herb of the genus Glycyrrhiza genus in the leguminous family. It is one of the commonly used Chinese medicinal materials in clinical practice and is known as "ten directions and nine herbs". According to the theory of traditional Chinese medicine, the traditional Chinese medicine licorice has a flat taste and a sweet taste, which can harmonize the urgency, moisten the lungs, detoxify, harmonize various medicines, and nourish the heart and spleen; modern pharmacological effects show that licorice has anti-oxidation, anti-tumor, anti-inflammatory, anti-aging, liver protection, Lowering blood sugar, regulating the body's immunity and other effects. Licochalcone A is a flavonoid compound characteristically accumulated in Glycyrrhiza inflate. Pharmacological studies have shown that i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C49/84C07C45/78C07C45/79A01G22/00A01G22/40A01G7/06
CPCC07C45/78C07C45/79A01G22/00A01G22/40A01G7/06C07C49/84
Inventor 王瑛李勇青周丽君曾江艺
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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