Application of selenized galactomannan in preparation of oxidative stress injury resisting agent

A galactomannan, anti-oxidative stress technology, applied in the direction of antitoxins, medical preparations containing active ingredients, drug combinations, etc. Hydrogen damage and other issues, to protect cells from oxidative stress damage, improve vitality, and improve antioxidant capacity

Active Publication Date: 2021-06-15
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no report in the prior art that selenized galactomannan protects macrophages from hydrogen peroxide damage

Method used

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  • Application of selenized galactomannan in preparation of oxidative stress injury resisting agent
  • Application of selenized galactomannan in preparation of oxidative stress injury resisting agent
  • Application of selenized galactomannan in preparation of oxidative stress injury resisting agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The extraction and selenization modification of galactomannan comprises the following steps:

[0029] (1) Extraction of galactomannan

[0030] The leguminous seeds containing galactomannan (Tianjing) were mechanically crushed to 20-100 mesh, added distilled water at a solid-to-liquid ratio of 1:50, extracted at 50°C for 24 hours, and centrifuged at 10,000 rpm for 10 minutes , to obtain a supernatant, adding absolute ethanol to the supernatant to obtain a precipitate, and vacuum drying the precipitate to obtain a galactomannan (GM) powdery solid, using gel chromatography to measure the molecular weight of the galactomannan, and Its purity was determined by ion chromatography.

[0031] (2) Selenization modification of galactomannan using the nitric acid-sodium selenite method

[0032] First, 1% galactomannan was added to a three-necked flask containing 0.5% (v / v) sodium nitrate solution, and stirred at 150 rpm for 2 h to completely dissolve GM. Subsequently, sodium sel...

Embodiment 2

[0048] Selenated galactomannan reacts with hydrogen peroxide (H 2 o 2 )-induced protective effect on cell injury

[0049] (1) Toxicity of selenized galactomannan to cells

[0050] The toxicity test of six SeGM and unmodified GM to RAW 264.7 cells was determined by co-cultivating the cells with samples of 7 different concentrations (25, 50, 100, 200, 400, 800 μg / mL) for 24 h, and the cell survival Rates were determined by CCK-8. Dissolve SeGM in water to prepare the above-mentioned solutions with different concentrations.

[0051] (2)H 2 o 2 Toxic to cells

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Abstract

The invention discloses application of selenized galactomannan in preparation of an oxidative stress injury resisting agent, and belongs to the technical field of macromolecular polysaccharides. The selenized galactomannan is obtained after galactomannan is treated by a nitric acid-sodium selenite method. The selenized galactomannan can obviously improve the cell viability of macrophages after H2O2 injury, improve the activity of superoxide dismutase and reduce the content of malondialdehyde, and has an obvious effect of protecting cells in a body from oxidative stress injury. The application lays a theoretical foundation for activity analysis of a galactomannan selenized product and development of an antioxidant product.

Description

technical field [0001] The invention belongs to the technical field of polymer polysaccharides, and in particular relates to the application of selenized galactomannans in the preparation of anti-oxidative stress damage agents. Background technique [0002] In recent years, galactomannan has attracted more attention because of its excellent immunoregulatory effect and enhanced antioxidant capacity, among which galactomannan has a more prominent immunoregulatory effect. In vitro experiments have shown that galactomannan can Promotes the activation of human monocyte-derived dendritic cells. The biological activity of polysaccharides is closely related to its molecular structure. Therefore, proper modification and introduction of new functional groups may change the physicochemical properties, three-dimensional structure and the resulting biological activity of galactomannan. Examples of galactomannan modifications in the prior art include sulfated galactomannan and galactoma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/736A61P39/06C08B37/00
CPCA61K31/736C08B37/0087A61P39/06
Inventor 张晓彤勇强黄曹兴陶昱恒陈雨姝廖吉丽董昊路李鑫赖晨欢
Owner NANJING FORESTRY UNIV
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