Kit and detection system for detecting anti-cyclic citrullinated peptide antibody

Abstract

The embodiment of the invention relates to a kit and detection system for detecting an anti-cyclic citrullinated peptide antibody. The kit comprises a shell, reagents and an information card; the reagent and the information card are arranged in the shell; standard curves of absorbance difference values and concentration values are stored in the information card; the reagents comprise a first reagent, a second reagent and a third reagent; the second reagent comprises latex particles coated with a first recognition site antigen of the anti-cyclic citrullinated peptide; the third reagent comprises latex particles coated with a second recognition site antigen of the anti-cyclic citrullinated peptide; the second reagent and the third reagent both comprise a sealing agent and a suspending aid. According to the kit, the two kinds of latex particles coated with anti-cyclic citrullinated peptide recognition site antigens are adopted, so that the reaction intensity of antigens and antibodies is enhanced. The reagents contain the suspending aid, the suspension stability of the latex particles is strong, the anti-cyclic citrullinated peptide antibody can be more effectively adsorbed; and in addition, the dispersion stability of the latex particles can be maintained by combining the suspending aid with the sealing agent, so that the accuracy of the kit is high.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of medical detection, in particular to a kit and a detection system for detecting anti-cyclic citrullinated peptide antibodies. Background technique [0002] Anti-cyclic citrullinated peptide antibodies (anti-CCP antibodies) are polypeptide fragments of cyclic polyguanidine. Anti-CCP antibodies are spontaneously secreted by B lymphocytes in patients with rheumatoid arthritis (RA), while B lymphocytes in patients with other diseases and normal people do not spontaneously secrete anti-CCP antibodies. Anti-CCP antibody has high sensitivity and specificity to rheumatoid arthritis (RA), and is a highly specific indicator for the early diagnosis of rheumatoid arthritis (RA). At present, it is believed that the diagnostic sensitivity of anti-CCP antibody to rheumatoid arthritis (RA) is 50%-78%, the specificity is 96%, and the positive rate of early-stage patients can reach 80%. In additi...

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Problems solved by technology

[0004] However, in the process of realizing the embodiment of the present invention, the inventors of the present invention found that the enzyme-linked immunosorbent assay generally adopts manual operation, which is cumbersome and inefficient, and has large human influence factors, so the results can only be measured qualitatively or imprecisely.

The single charge of electrochemiluminescence immunoassay is relatively high, so the above two detection methods are difficult to popularize and apply

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