Induced Human Pluripotent Stem Cell Directed Endothelial Differentiation Method

Abstract

The present invention relates to a method for inducing directional endothelial differentiation of human pluripotent stem cells, which specifically includes the following steps: 1. When the human induced pluripotent stem cells reach 80% confluence or more, use a differentiation medium containing 10-50ng / ml WNT3a to digest the pluripotent stem cells after digestion. 2. Use the differentiation medium containing 10-50ng / ml WNT3a and 10-30ng / ml BMP2 to induce differentiation of the cells; 3. Use the differentiation medium containing 10-30ng / ml BMP2, In step 2, the induced and differentiated cells are further induced to differentiate into mesoderm cells; 4. The cells are further induced to differentiate into endothelial cells by using a medium containing 10-15ng / ml FGF8. By improving the culture medium and related cytokines in the present invention, the experimental scheme can have higher endothelial induction efficiency.

Description

technical field

[0001] The invention relates to the field of directional differentiation of induced human pluripotent stem cells, in particular to a method for inducing directional endothelial differentiation of human pluripotent stem cells. Background technique

[0002] Cardiovascular disease (CVD), often caused by endothelial dysfunction, is one of the leading causes of death worldwide. Direct endothelial replacement therapy for CVD is hampered by the lack of methods to isolate sufficient numbers of functioning autologous endothelial cells (ECs) for transplantation, and our limited understanding of how endogenous ECs are dysfunctional in the development of various cardiovascular diseases . Innovations in human induced pluripotent stem cells (HiPSCs), cells reprogrammed from somatic cells to a pluripotent state similar to embryonic stem cells (ESCs), can generate large numbers of patient-specific ECs that can be used for transplantation, drug screening or research to explo...

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