A method for detecting gene chip hybridization result

A technology for detecting gene chips and gene chips, which is applied in the field of determination of biochip hybridization results, can solve problems such as difficulty in weak signal detection, achieve the effects of reducing background interference, improving detection throughput, and improving detection capabilities

Active Publication Date: 2021-07-30
BEIJING BIONAXIN BIOTECH CO LTD
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Problems solved by technology

[0008] Another object of the present invention is to provide a method for detecting the hybridization result of a gene chip, which overcomes the inherent deficiency of difficulty in detecting weak signals caused by background interference in fluorescence intensity imaging

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  • A method for detecting gene chip hybridization result
  • A method for detecting gene chip hybridization result

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Embodiment Construction

[0039] Exemplary embodiments of the present disclosure will be described in detail below with reference to the accompanying drawings, wherein the same or similar reference numerals represent the same or similar elements. In addition, in the following detailed description, for purposes of explanation, numerous specific details are set forth in order to provide a comprehensive understanding of the embodiments of the present disclosure. It may be evident, however, that one or more embodiments may be practiced without these specific details. In other instances, well-known structures and devices are shown in diagrammatic form to simplify the drawings.

[0040] The invention provides a method for detecting the hybridization result of a gene chip. The method comprises: constructing a gene chip detector, the detector including a pulsed laser light source system and a collection system for fluorescence lifetime imaging; hybridizing the gene chip with the sample to be detected and compl...

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Abstract

The invention discloses a method for detecting the hybridization result of a gene chip, comprising: constructing a gene chip detector; placing the substrate of the gene chip after hybridization and fluorescent labeling on a packaged microchannel plate; turning on a pulse laser light source system, emitting The pulsed laser is emitted to make the fluorescent substance on the probe with fluorescent labeling group on the substrate of the gene chip reach an excited state; the photons released after the fluorescent substance on the probe is excited are converted into The electrical signal is detected; the cumulative signal data of the probes at different times and channel positions are counted, and the fluorescence lifetime curve of each probe is drawn. The method for detecting the hybridization result of the gene chip adopts the time-correlated single-photon counting method to carry out repeated detection more than 100,000 times, and realizes the drawing of the fluorescence lifetime curve, thereby improving the detection sensitivity of the hybridization result of the gene chip.

Description

technical field [0001] The invention relates to the field of assay methods for biochip hybridization results, in particular to a assay method for detecting gene chip hybridization results, which uses a method for detecting probe chip hybridization including nucleic acids. The invention also particularly relates to an in vitro diagnostic testing instrument, which belongs to the time-resolved fluorescence detection and analysis instrument in the in vitro diagnostic testing instrument, and also relates to a molecular biological information analysis and processing system in the molecular diagnostic testing instrument. Background technique [0002] Biochip technology has been widely used in clinical disease diagnosis, health management, drug research and development, animal and plant quarantine, food testing, environmental monitoring, scientific research, forensic testing and many other fields. It has broad application prospects and a very large market demand. [0003] Gene chip ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6408G01N21/6458
Inventor 华子昂刘宝全竹添朱美瑛万君兴张建李娜
Owner BEIJING BIONAXIN BIOTECH CO LTD
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