Up-conversion luminescence immunochromatographic test strip for quantitative detection of O-type foot-and-mouth disease virus antibody and preparation method thereof
A technology of foot-and-mouth disease virus and chromatography test paper, which is applied in the field of material chemistry and immunology detection, can solve the problems of time-consuming and other problems, and achieve the effect of good specificity and repeatability, high sensitivity and good stability
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Embodiment 1
[0043] Embodiment 1 Preparation of up-conversion luminescence immunochromatography test strip
[0044] 1. Preparation and modification of upconversion luminescent nanomaterials
[0045] 1) Synthesis of upconversion luminescent nanomaterials
[0046] a NaYF 4 : 18%Yb, 2%Er nanocrystal synthesis process is as follows: Weigh YCl 3 ·6H 2 O 0.2366g, YbCl 3 ·6H 2 O 0.0775g and ErCl 3 ·6H 2 O 0.0076g was added to a 100mL round bottom flask and mixed with 6mL oleic acid (OA) and 15mL octadecene (ODE). Heated to 160°C under nitrogen protection to form a homogeneous solution and then cooled to room temperature. Slowly add 10mL containing 0.1g NaOH and 0.148g NH 4 F in methanol solution. The reaction solution was slowly heated to 50°C for 40 minutes, and then heated to 100°C for 40 minutes to remove methanol and water. Then, under the protection of nitrogen, it was heated to 320° C. for 1.5 h. After the solution was naturally cooled, ethanol was added to precipitate and washe...
Embodiment 2
[0070] Application Evaluation of Example 2 Up-conversion Luminescence Immunochromatography Test Strip
[0071] 1. Quantitative immunochromatographic method for O-type foot-and-mouth disease virus antibody
[0072] The O-type foot-and-mouth disease antibody was diluted with diluent to different concentrations of 0.1, 0.3, 1, 3, 30, 50 μg / mL. Add different concentrations of antibodies into the sample wells of the up-conversion luminescence immunochromatography test strip, and after 20 minutes, detect on the tester and record the results. The concentration and signal ratio were plotted logarithmically to establish a linear standard curve. Each batch of immunochromatographic test paper needs to correspond to a standard curve. The test results of different concentrations of O-type foot-and-mouth disease antibodies are as follows: image 3 .
[0073] 2. Sensitivity
[0074] The concentration and signal value of type O foot-and-mouth disease antibody were taken logarithmically and...
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