Up-conversion luminescence immunochromatographic test strip for quantitative detection of O-type foot-and-mouth disease virus antibody and preparation method thereof

A technology of foot-and-mouth disease virus and chromatography test paper, which is applied in the field of material chemistry and immunology detection, can solve the problems of time-consuming and other problems, and achieve the effect of good specificity and repeatability, high sensitivity and good stability

Active Publication Date: 2021-06-01
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, ELISA, the most common method for FMD diagnosis, has high sensitivity, but it takes a long time, usually more than 1 hour to collect data
Colloidal gold immunochromatography has been applied in different fields, but it can only perform qualitative or semi-quantitative detection, so it has great limitations

Method used

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  • Up-conversion luminescence immunochromatographic test strip for quantitative detection of O-type foot-and-mouth disease virus antibody and preparation method thereof
  • Up-conversion luminescence immunochromatographic test strip for quantitative detection of O-type foot-and-mouth disease virus antibody and preparation method thereof
  • Up-conversion luminescence immunochromatographic test strip for quantitative detection of O-type foot-and-mouth disease virus antibody and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 Preparation of up-conversion luminescence immunochromatography test strip

[0044] 1. Preparation and modification of upconversion luminescent nanomaterials

[0045] 1) Synthesis of upconversion luminescent nanomaterials

[0046] a NaYF 4 : 18%Yb, 2%Er nanocrystal synthesis process is as follows: Weigh YCl 3 ·6H 2 O 0.2366g, YbCl 3 ·6H 2 O 0.0775g and ErCl 3 ·6H 2 O 0.0076g was added to a 100mL round bottom flask and mixed with 6mL oleic acid (OA) and 15mL octadecene (ODE). Heated to 160°C under nitrogen protection to form a homogeneous solution and then cooled to room temperature. Slowly add 10mL containing 0.1g NaOH and 0.148g NH 4 F in methanol solution. The reaction solution was slowly heated to 50°C for 40 minutes, and then heated to 100°C for 40 minutes to remove methanol and water. Then, under the protection of nitrogen, it was heated to 320° C. for 1.5 h. After the solution was naturally cooled, ethanol was added to precipitate and washe...

Embodiment 2

[0070] Application Evaluation of Example 2 Up-conversion Luminescence Immunochromatography Test Strip

[0071] 1. Quantitative immunochromatographic method for O-type foot-and-mouth disease virus antibody

[0072] The O-type foot-and-mouth disease antibody was diluted with diluent to different concentrations of 0.1, 0.3, 1, 3, 30, 50 μg / mL. Add different concentrations of antibodies into the sample wells of the up-conversion luminescence immunochromatography test strip, and after 20 minutes, detect on the tester and record the results. The concentration and signal ratio were plotted logarithmically to establish a linear standard curve. Each batch of immunochromatographic test paper needs to correspond to a standard curve. The test results of different concentrations of O-type foot-and-mouth disease antibodies are as follows: image 3 .

[0073] 2. Sensitivity

[0074] The concentration and signal value of type O foot-and-mouth disease antibody were taken logarithmically and...

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Abstract

The invention discloses an up-conversion luminescent immunochromatographic test strip for quantitative detection of O-type foot-and-mouth disease virus antibody and a preparation method thereof. The present invention utilizes Escherichia coli prokaryotic expression technology to express O-type foot-and-mouth disease (O-FMDV) capsid protein, and assembles O-type foot-and-mouth disease virus-like particles in vitro as capture antigens; utilizes the special luminescent properties of up-conversion luminescent nanomaterials, and uses them as fluorescent The label is coupled with protein G, and then combined with the antibody to prepare the up-conversion luminescent immunochromatographic test strip of the present invention. The immunochromatographic test paper prepared by the invention has high sensitivity, good specificity and repeatability, and stable results, can be applied to the monitoring of O-type foot-and-mouth disease antibody level, and helps to further understand the status of O-type foot-and-mouth disease antibody in cloven-hoofed livestock.

Description

technical field [0001] The present invention relates to an up-conversion luminescence immunochromatography test strip made based on an up-conversion luminescence nanomaterial and a preparation method thereof, and also relates to the application of the up-conversion luminescence immunochromatography test strip in the quantitative detection of O-type foot-and-mouth disease virus antibody application. The invention belongs to the field of material chemistry and immunology detection. Background technique [0002] The antigens of foot-and-mouth disease virus mainly include the whole virus and its structural proteins. Due to the incomplete inactivation of the complete virus, there is a risk of infection during use; although some structural proteins (such as VP1) are immunogenic, they have certain differences in structure from the complete virus, and the immune response The effect is difficult to guarantee. Virus-like particles (VLPs) are empty capsids without RNA, have the same...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/569G01N33/558C09K11/85C09K11/02
CPCC09K11/025C09K11/7773G01N33/558G01N33/56983G01N33/582G01N33/6854G01N2333/09G01N2469/20
Inventor 郭慧琛孙世琪侯风萍张韵白满元柳海云高震东殷宏
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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