Micro-nano fluidic chip-based urine visible component analyzer and use method thereof

Abstract

The invention discloses a micro-nano fluidic chip-based urine visible component analyzer and a use method thereof, and the use method comprises the following steps: S1, sucking a urine sample through a sucking device, and injecting the urine sample into a channel center position of a particle imaging chamber; s2, keeping the sheath fluid at the same flow speed on the two sides of the urine sample, and adjusting the posture of urine particles by adjusting the flow of the sheath fluid on the single side; s3, in the flowing process of the urine sample, using a stroboscope lamp for carrying out staged irradiation on the sample plate, and matching a proper sample flow rate so that an optical image of urinary sediment particles is obtained; s4, amplifying the urinary sediment particle optical image, and shooting through a camera to obtain a plurality of electronic images; s5, classifying and counting the multiple electronic images so that the number of particles of all components in the urine sample is obtained. According to the method, particle sedimentation which can only be achieved by a common instrument within two seconds in one minute is achieved, and the urine visible component analysis efficiency is greatly improved.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic medical equipment, in particular to a urine formed component analyzer based on a micro-nano fluidic chip and a method for using the same. Background technique [0002] At present, domestic manufacturers of urine analyzers include Guilin Youlit, Changsha Aiwei, Chongqing Tianhai, etc. These manufacturers mainly adopt the second-generation urine formed component analysis technology: sedimentation urine formed component analysis principle, simulated manual microscope inspection, Inhale the urine sample into the sedimentation tank through instruments and equipment, wait for the formed components of the urine sample to settle naturally, the microscopic imaging system will take pictures of the sedimentation tank, and classify and count various formed components through the recognition software, so as to achieve Purpose of screening analysis of urine samples. [0003] After the urine sample settles na...

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Problems solved by technology

[0003] After the urine sample settles naturally in the settling tank, an electron microscope is used to directly photograph the urine formed component sample in the settling tank. This design is affected by the sedimentation principle, and the urine formed component cannot be completely settled on the same plane, and the center deviation range of the formed component sample is within At about 40 microns, the imaging resolution at different positions varies greatly, resulting in the inability of the classification algorithm to accurately classify the components, resulting in low accuracy and repeatability of the equipment

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