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Application of BACE2 inhibitor in preparation of medicine for treating eye melanoma

A melanoma and inhibitor technology, applied in the field of ocular melanoma treatment, can solve the problems of unclear curative effect and mechanism of action of ocular melanoma

Active Publication Date: 2021-07-06
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the application of BACE2 inhibitor BACE2 Inhibitor 3l in ocular melanoma, and its efficacy and mechanism of action on ocular melanoma are still unclear

Method used

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  • Application of BACE2 inhibitor in preparation of medicine for treating eye melanoma
  • Application of BACE2 inhibitor in preparation of medicine for treating eye melanoma
  • Application of BACE2 inhibitor in preparation of medicine for treating eye melanoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Cell Proliferation Experiment (CCK8)

[0022] Cell Proliferation Assay (CCK8)

[0023] Experimental materials: ocular melanoma cell line OCM1 was provided by Professor John F. Marshall (Tumor Biology Laboratory, UK Cancer Research Clinical Center, John Vane Science Center, London, UK), CRMM1 was provided by Professor MartineJ. Jager (Leiden University Medical Center, Leiden, the Netherlands) ), trypsin was purchased from Gibco, CCK8 was purchased from Nippon Dojin Chemical, and BACE2Inhibitor 3l was purchased from Axon Medchem.

[0024] Experimental steps: trypsinize the ocular melanoma cell lines OCM1 and CRMM1, centrifuge at 800rpm, remove the supernatant, add fresh culture medium, count the number of cells with an electronic cell counting board, and plant them in In a 96-well cell culture plate, 100uL culture medium per well, set the drug concentration gradient according to 0, 1, 2.5, 5umol / LBACE2 Inhibitor 3l, and set up 3 duplicate wells, fully mix the c...

Embodiment 2

[0025] Embodiment 2 plate cloning experiment

[0026] Experimental materials: ocular melanoma cell lines OCM1 and CRMM1, trypsin and PBS were purchased from Gibco, BACE2 Inhibitor 3l was purchased from Axon Medchem, and crystal violet was purchased from Biyuntian

[0027] Experimental steps: Select ocular melanoma cell lines OCM1 and CRMM1 in logarithmic growth phase and in good condition, digest the cells with trypsin when they grow to about 70-80%, count them with a cell counting plate, and plant them in a 6-well plate Inside, make each well contain 2mL culture medium, 1000 cells, and respectively contain 0, 1, 2.5, 5umoL BACE2 Inhibitor 3l drug concentration. Since the required amount of cells in this part of the experiment is relatively small, the volume of the cell suspension added will be particularly small. You can first dilute the cells to 10000 / mL, and then take 10uL of the cell suspension to add. Post-placed at 37°C, 5% CO 2 Culture in the cell incubator for up to ...

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Abstract

Aiming at the condition that the eye melanoma is lack of an effective drug for treatment, the invention firstly discovers that BACE2Inhibitor3l can obviously inhibit the growth of eye melanoma cells, and the drug effect is increased along with the increase of concentration. The invention provides a new treatment medicine for clinical treatment of eye melanoma, which improves the treatment effectiveness, prolongs the life of a patient, and improves the life quality. The invention develops a new field of the application of the BACE2Inhibitor3l in the treatment of the eye melanoma.

Description

technical field [0001] The invention relates to the technical field of ocular melanoma treatment, in particular to the application of BACE2 inhibitors in the preparation of medicaments for treating ocular melanoma. Background technique [0002] Ocular melanoma includes uveal melanoma (Uveal melanoma, UM) and conjunctival melanoma (Conjunctival melanoma, CM). Among them, UM is the most common intraocular malignancy in adults, with a high degree of malignancy. About 50% of UM patients develop liver metastases, and the median survival time of patients with liver metastases is only 10.2 months. The onset of CM is relatively insidious, and often does not affect vision in the early stage. When it is found, it often spreads and metastasizes, and the prognosis is poor, which seriously affects the life and health of patients. At present, the main treatment methods for ocular melanoma are surgery and radiotherapy and chemotherapy, but it is not sensitive to conventional radiotherapy ...

Claims

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Application Information

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IPC IPC(8): A61K31/166A61P35/00A61P27/02
CPCA61K31/166A61P35/00A61P27/02
Inventor 柴佩韦何芳邻贾仁兵范先群
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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