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Method for detecting antiepileptic drugs and metabolites thereof in hair by liquid chromatography-mass spectrometry

A technology for anti-epileptic drugs and metabolites, which can be used in measurement devices, instruments, scientific instruments, etc., to solve problems such as difficulties, low content of anti-epileptic drugs, and complex hair components, and achieve the effect of sensitive detection.

Pending Publication Date: 2021-07-09
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the composition contained in the hair is very complex, and the content of antiepileptic drugs (or their metabolites) in the hair of people taking antiepileptic drugs is extremely low
How to effectively separate antiepileptic drugs (or their metabolites) from other interfering components in the hair if the above liquid chromatography-mass spectrometry method is used to detect hair samples is a very difficult problem

Method used

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  • Method for detecting antiepileptic drugs and metabolites thereof in hair by liquid chromatography-mass spectrometry
  • Method for detecting antiepileptic drugs and metabolites thereof in hair by liquid chromatography-mass spectrometry
  • Method for detecting antiepileptic drugs and metabolites thereof in hair by liquid chromatography-mass spectrometry

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Embodiment 1

[0040] Example 1, detection of levetiracetam, lamotrigine and oxcarbazepine metabolite content in hair

[0041] (1) Establishment of standard curves for antiepileptic drugs

[0042] a, preparation of reference substance solution: weigh respectively 0.2mg levetiracetam and lamotrigine, 0.5mg oxcarbazepine metabolite (10-hydroxyl-carbamazepine) standard reference substance, levetiracetam plus Dissolve in 1ml of water, dissolve lamotrigine in 1ml of DMSO, dissolve oxcarbazepine metabolite 10-hydroxy-carbamazepine in 1ml of DMSO, add 7ml of diluent (20% acetonitrile + 80% water), and mix to obtain levetirazil Tan and lamotrigine 20ug / ml, oxcarbazepine metabolite 10-hydroxy-carbamazepine 50ug / ml mixed grade I working solution reference substance solution;

[0043]b. Preparation of series concentration standard working solution: take step a reference substance solution, add diluent (20% acetonitrile+80% water) to prepare series concentration reference substance solution, add blank ...

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Abstract

The invention belongs to the technical field of detection of drugs and metabolites thereof and particularly relates to a method for detecting antiepileptic drugs and metabolites thereof in hair by liquid chromatography-mass spectrometry. The invention provides a system which is used for judging whether the dosage of an anti-epileptic drug is excessive or insufficient by detecting or inputting the content of the anti-epileptic drug or metabolites thereof in hair. The detection method comprises the following steps of (1) pretreating a hair sample to obtain a solution to be detected; and (2) detecting the concentration of the anti-epileptic drug in the hair by adopting a liquid chromatography-mass spectrometry method. The detection method is simple, convenient and sensitive, the long-term medication condition of a subject can be reflected by detecting the anti-epileptic medicine in the hair, the hair sample is simple and convenient to take, the storage requirement is low, and the method is suitable for clinical large-scale popularization.

Description

technical field [0001] The invention belongs to the technical field of detection of drugs and their metabolites, in particular to a method for detecting antiepileptic drugs and their metabolites in hair by liquid mass spectrometry. Background technique [0002] Epilepsy is a common chronic neurological disease and is mainly treated with antiepileptic drugs. It is particularly important to control epileptic seizures when a sufficient amount of treatment is administered rationally. At present, the concentration of plasma / serum antiepileptic drugs (or their metabolites) in patients is mainly detected through venous blood sampling, but venous blood sampling is invasive and only reflects the current drug concentration level in the body. For special groups such as those with special diseases who have difficulty in blood collection, children, pregnant women, and the elderly, it is more necessary to regularly go to the hospital to draw blood to measure drug concentration. Therefore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/7266
Inventor 陈蕾李宛凌
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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