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Application of Bifidobacterium bifidum tmc3115 and its complex bacteria in alleviating and improving allergy

A technology of bifidobacteria bifidum and compound lactic acid bacteria, applied in the field of lactic acid bacteria, can solve problems such as adverse side effects, achieve the effects of avoiding side effects, promoting immune response, and blocking the secretion of IgE

Active Publication Date: 2022-02-11
河北一然生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention proposes the application of Bifidobacterium bifidum TMC3115 and its complex bacteria in alleviating and improving allergy, and solves the problem that the medicine for allergy treatment in the prior art will produce adverse side effects

Method used

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  • Application of Bifidobacterium bifidum tmc3115 and its complex bacteria in alleviating and improving allergy
  • Application of Bifidobacterium bifidum tmc3115 and its complex bacteria in alleviating and improving allergy
  • Application of Bifidobacterium bifidum tmc3115 and its complex bacteria in alleviating and improving allergy

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Experimental program
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Effect test

Embodiment 1

[0036] Example 1: Cell experiment

[0037] 1. Cell culture:

[0038] After J774A.1 (mouse mononuclear macrophage) cells were recovered, they were placed in a culture flask containing DMEM complete culture solution, and placed at 37°C, 5% CO 2Incubate in an incubator, wait for the cells to grow well and reach a density of 80%, then subculture (48h), and pass three times for testing.

[0039] 2. Test strains:

[0040] Lactobacillus plantarum LP45, Lactobacillus acidophilus La28, Bifidobacterium bifidum TMC3115, compound lactic acid bacteria: The mass ratio of Lactobacillus plantarum LP45, Lactobacillus acidophilus La28, and Bifidobacterium bifidum TMC3115 is 2:5:3, with Lactobacillus rhamnosus LGG, which proved to have anti-allergic effect, was used as a control.

[0041] 3. Preparation of bacterial suspension

[0042] The above five groups of lactic acid bacteria were subcultured for 3 times, cultured at 37°C for 18 hours, centrifuged at 5000r / min at 4°C for 5min, and the s...

Embodiment 2

[0049] Embodiment 2: Animal experiment 1

[0050] Six-week-old male SPF grade BABL / c mice were purchased from Beijing Weitong Lihua Company. The animal experiment protocol was approved by the Ethics Committee of Jiangnan University (JN.No20200710b0701020) and implemented in accordance with EU guidelines (2010 / 63 / EU). The feeding environment was maintained at (23±2)°C, relative humidity (50±10)%, and 12 hours of light and 12 hours of night alternated. 120 mice were randomly divided into blank group, model group, La28 group, LP45 group, TMC3115 group and compound lactic acid bacteria group (the mass ratio of Lactobacillus plantarum LP45, Lactobacillus acidophilus La28 and Bifidobacterium bifidum TMC3115 was 2:5 :2), hereinafter referred to as composite group. The mice were fed with 0.2 mL of normal saline solution from the 8th day to the 28th day, and the La28 group, LP45 group, TMC3115 group and the compound group were fed with 0.2 mL of normal saline solution. Bacteria powd...

Embodiment 3

[0071] Embodiment 3: Animal experiment 2

[0072] Six-week-old male SPF grade BABL / c mice were purchased from Beijing Weitong Lihua Company. The animal experiment protocol was approved by the Ethics Committee of Jiangnan University (JN.No20200710b0701021) and implemented in accordance with EU guidelines (2010 / 63 / EU). The feeding environment was maintained at (23±2)°C, relative humidity (50±10)%, and 12 hours of light and 12 hours of night alternated. 120 mice were randomly divided into blank group, model group, La28 group, LP45 group, TMC3115 group and compound lactic acid bacteria group (the mass ratio of Lactobacillus plantarum LP45, Lactobacillus acidophilus La28 and Bifidobacterium bifidum TMC3115 was 3:1 : 2) Hereinafter referred to as the composite group. After 7 days of acclimatization, the mice began to be gavaged. From the 8th day to the 35th day, the blank group and the model group were gavaged with 0.2mL physiological saline solution, and the La28 group, LP45 grou...

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Abstract

The present invention relates to the technical field of lactic acid bacteria, and proposes a compound lactic acid bacterium, including Bifidobacterium bifidum TMC3115, Lactobacillus plantarum LP45, and Lactobacillus acidophilus La28. A compound lactic acid bacteria preparation, comprising compound lactic acid bacteria and additives, wherein the additives include 20 to 50 parts of fructooligosaccharides, 20 to 50 parts of galactooligosaccharides, 0.1 to 3 parts of zinc gluconate, and 1 to 10 parts of calcium lactate according to the mass ratio. ferrous lactate, 0.1-3 parts of ferrous lactate, 0.001-0.5 parts of vitamin A, 0.1-3 parts of vitamin C, 5-25 parts of collagen peptide, 0.1-1 part of euglena, 5-10 parts of blueberry powder, and euglena Algae 0.01~0.1 part. Through the above technical solution, the problem that the drugs for allergy treatment in the prior art can produce adverse side effects is solved.

Description

technical field [0001] The invention relates to the technical field of lactic acid bacteria, in particular to the application of Bifidobacterium bifidum TMC3115 and its complex bacteria in alleviating and improving allergy. Background technique [0002] In healthy people, the number of immune cells Th1 and Th2 is balanced, while the number of Th2 in the body of allergic people is higher than the number of Th1, resulting in an imbalance of the two types of immune cells. Cytokines secreted by Th2 cells, such as interleukins IL-4 and IL-13, play an important role in allergic inflammation. IL-4 is considered to be the most representative inflammatory factor of Th2 cells, and IL-4 can promote the secretion of IgE, so increased IL-4 concentration is an important feature of allergic patients. [0003] For certain allergic diseases, such as allergic rhinitis, atopic dermatitis, medical practice tends to focus on treating the disease and reducing symptoms, but quality of life is als...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135C12R1/01C12R1/25C12R1/23
CPCC12N1/20A23L33/135A23V2002/00A23V2400/517A23V2200/30
Inventor 鄢梦洁郭润晴路江浩雷敏杨玲霍文敏李玲高景伟赵林森何方
Owner 河北一然生物科技股份有限公司
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