A kind of identification method and application of bee pollen allergenic protein

An identification method and technology of allergenic proteins, which can be used in instruments, measuring devices, scientific instruments, etc., and can solve problems such as imperfect identification of allergenic proteins.

Active Publication Date: 2021-11-16
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method solves the technical problem of imperfect identification of allergenic proteins in bee pollen

Method used

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  • A kind of identification method and application of bee pollen allergenic protein
  • A kind of identification method and application of bee pollen allergenic protein
  • A kind of identification method and application of bee pollen allergenic protein

Examples

Experimental program
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Effect test

Embodiment 1

[0059] This embodiment provides a method for identifying rape bee pollen allergens, and the specific steps are as follows:

[0060] 1) Sample pretreatment to obtain bee pollen freeze-dried powder:

[0061] Accurately weigh 5.0g of ground rapeseed bee pollen powder (100-300 mesh);

[0062] The bee pollen powder is subjected to vacuum freeze-drying by using a vacuum freeze-drying machine, and the required drying time is about 48 hours to obtain the bee pollen freeze-dried powder.

[0063] Use GB5009.3-2016 "National Food Safety Standard for Determination of Moisture in Food" to measure the moisture content of the freeze-dried powder, which meets the standard below 7%.

[0064] 2) Protein extraction to obtain precipitated protein:

[0065] Accurately weigh 100.0 mg rape bee pollen freeze-dried powder, add 1 mL protein extractant;

[0066] Ultrasonic crushing, the condition is ultrasonic 3s, interval 3s, repeat 30 times, continue to shake at room temperature for 30~60min after ...

Embodiment 2

[0099] This example provides an identification method for known allergenic proteins in rapeseed bee pollen to verify the accuracy of the identification method described in the present invention.

[0100] The specific experimental steps are the same as in Example 1. After identification, a known allergenic protein in rape bee pollen is Polcalcin. At the same time, the primary sequence of Polcalcin is obtained, and the T cells and T cells of the allergenic protein are obtained through structural analysis. B cell antigen epitopes were positive, consistent with the characteristics of known allergenic proteins.

[0101] It can be seen that the above test results have verified that the identification method of the present invention is accurate and reliable.

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Abstract

The invention relates to the technical field of analysis and detection, in particular to an identification method and application of a bee pollen allergenic protein. The method for identifying allergenic proteins in bee pollen provided by the present invention comprises: taking bee pollen freeze-dried powder as an identification sample, carrying out protein extraction, proteolysis, peptide desalination and mass spectrometry detection and identification on the sample; During the extraction, the mixture of the sample and the protein extractant was treated with intermittent ultrasonic crushing. The identification method provided by the invention solves the current situation of incomplete identification of bee pollen allergens; at the same time, the identification method of the invention obtains the amino acid sequence of the potential allergen of bee pollen, and analyzes its recognition by T cells and B cells Antigenic epitope, clear allergenic properties. The identification method provided by the present invention is applicable to bee pollen from different plant sources, especially through the identification method, five potential allergenic proteins in rape bee pollen are further clarified, which provides a basis for subsequent quality and safety control.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to an identification method and application of a bee pollen allergenic protein. Background technique [0002] Because of its rich nutritional value, high edible and medicinal effects, bee pollen has been used in food, health care products, pharmaceutical industries, and animal husbandry. With the expansion of the scope of application of bee pollen, the safety problems brought about by eating bee pollen have gradually attracted people's attention. [0003] Bee pollen is derived from plant pollen. Although some allergens are changed or degraded after treatment by worker bees, and the allergenicity is improved compared with its plant source pollen, the data show that there are still some susceptible people who develop clinical symptoms after eating bee pollen. Allergy symptoms. [0004] At present, food allergen analysis methods mainly include enzyme-linked immunoassay...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/32G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/32G01N30/34G01N30/72G01N2030/324
Inventor 李强强吴黎明薛晓锋陶宇逍尹舒婷
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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