Carrier and testing method
A carrier and testing equipment technology, applied in the field of measurement accuracy, can solve the problems of unknown efficiency, impossibility to know the exact copy number of gene, and unknown exact copy number of target nucleic acid, so as to achieve the effect of accurate nucleic acid extraction efficiency
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[0156] The preparation and filling of the droplets is achieved, for example, by spraying a cell suspension containing a cell A in which the nucleic acid is incorporated into the nucleic acid a in the nucleus of the cell in the form of droplets, and making the droplets Landing on the base material in turn. "Spraying" means flying a cell suspension in the form of droplets. "Sequentially" means in sequence one after the other. "Falling" means causing a droplet to reach a base material.
[0157] The base material may be any one as long as it has a load portion made of a water-decomposable material; and specific examples thereof include the same base materials as those described in the above-mentioned "base material".
[0158] As the ejection device, a device for ejecting a cell suspension in the form of droplets (hereinafter, may also be referred to as a "spray head") can be suitably used.
[0159] Examples of methods for ejecting a cell suspension in the form of droplets inclu...
no. 1 approach 》
[0308] The test method of the present embodiment includes a process of calculating the efficiency of nucleic acid extraction from the cell A using the carrier (extraction efficiency calculation process).
[0309] In addition to the above-mentioned process, the test method of the present embodiment preferably further includes a process of determining the accuracy of the measured value of the gene by the genetic testing device according to the extraction efficiency (determination process A).
[0310] [Extraction efficiency calculation process]
[0311] In the extraction efficiency calculation process, first, nucleic acid is extracted from cell A using the carrier. Then, quantitative PCR was performed using the obtained nucleic acid to quantify the copy number of the nucleic acid. Next, the value obtained by dividing the quantitative value by the known copy number of nucleic acid present on the base material was calculated as the extraction efficiency of nucleic acid in percent....
no. 2 approach 》
[0324] In the case that the genetic testing device is a quantitative PCR device, the test method of the present embodiment includes a process of using a carrier to calculate the efficiency of nucleic acid extraction from cell A (extraction efficiency calculation process); by using two or more carriers (which have respectively The total copy number of nucleic acid contained in different vectors) amplifies nucleic acid using quantitative PCR equipment, creates a calibration curve, and then calculates the process of amplification efficiency (amplification efficiency calculation process); and determines the gene according to the extraction efficiency and amplification efficiency The process of the accuracy of the quantitative value (wherein the quantitative value is obtained using the quantitative PCR equipment) of the (determination process B).
[0325] In addition to the above-mentioned process, the test method of the present embodiment preferably further includes a process of ob...
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