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Application of m6A related lncRNA in preparation of product for predicting prognosis of colorectal cancer

A colorectal cancer and prognosis technology, applied in the field of biomedicine, can solve the problem of not being able to distinguish the benefits of patients with poor prognosis, and achieve good predictive performance

Pending Publication Date: 2021-10-15
THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these high-risk factors do not distinguish patients with a poor prognosis from those who benefit from chemotherapy

Method used

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  • Application of m6A related lncRNA in preparation of product for predicting prognosis of colorectal cancer
  • Application of m6A related lncRNA in preparation of product for predicting prognosis of colorectal cancer
  • Application of m6A related lncRNA in preparation of product for predicting prognosis of colorectal cancer

Examples

Experimental program
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Embodiment 1

[0036] (1) Obtaining patient information: Download early colorectal cancer RNA sequencing data (FPKM value) and corresponding clinical data, including age, gender, stage, overall survival time and survival status, from the Cancer Genome Atlas (TCGA) website. To reduce statistical bias, patients with no survival information or with a survival time of less than 3 months were excluded from further evaluation. After screening, 251 patients with early-stage colorectal cancer in the TCGA database were finally included in the analysis (see figure 1 ).

[0037](2) Acquisition of prognosis-related m6A-related lncRNAs: Using Ensembl's GTF files to distinguish mRNAs and lncRNAs in RNA-sequencing data of early colorectal cancer. Next, R software was used to extract the identified 23 m6A genes (METTTL3, METTTL14, METTTL16, WTAP, VIRMA, ZC3H13, RBM15, RBM15B, ALKBH5, FTO, YTHDC1, YTHDC2, YTHDF1, YTHDF2, YTHDF3, HNRNPC, FMR1, LRPPRC , HNRNPA2B1, IGFBP1, IGFBP2, IGFBP3, RBMX) expression dat...

Embodiment 2

[0041] Example 2 Detection of chemotherapeutic drug sensitivity

[0042] The Genomics of Cancer Drug Sensitivity (GDSC) database (https: / / cancerrxgene.org) is known to enable large-scale drug screening. Combined with genomic analysis, biomarkers of drug response can be systematically identified. Based on this database, the inventors used the "pRRophetic" package to predict the half-maximal inhibitory concentration (IC50) of commonly used chemotherapeutic drugs (camptothecin, cisplatin, rapamycin, methotrexate) in different risk groups of early-stage colorectal cancer patients ). Then, the Wilcoxon signed-rank test was used to compare the IC50 differences of different chemotherapeutic drugs in the high-risk group and the low-risk group in Example 1, and the drugs with significant differences between the groups were screened out, which were then used to guide the clinical implementation of the high-risk early stage results in the case 1 model. Postoperative chemotherapy in pat...

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Abstract

The invention discloses an application of m6A related lncRNA in preparation of a product for predicting colorectal cancer prognosis. According to the invention, lncRNAs related to m6A: AC087277.2, AC007991.4, AC008494.3, AC254629.1 and AL121583.1 are used as detection targets, and the constructed model is good in prediction performance; in clinical treatment, after patient diagnosis is clear, the constructed model can quickly judge the sensitivity of a high-risk early colorectal cancer patient to chemotherapy, a new treatment scheme is provided for postoperative chemotherapy of the early colorectal cancer patient, and important clinical significance and application prospects are achieved.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of m6A-related lncRNA in preparing a product for predicting the prognosis of colorectal cancer. Background technique [0002] Colorectal cancer (CRC) is one of the most common gastrointestinal malignancies and one of the leading causes of cancer-related death. Although the 5-year survival rate after surgery for early-stage CRC (stages I and II) can reach 90%, patients with stage I and II CRC still have a 5% and 20% risk of recurrence, respectively. Currently, the need for chemotherapy in patients with early-stage CRC mainly depends on known clinical and pathological risk factors, such as microsatellite instability, intestinal obstruction, etc. However, these high-risk factors did not differentiate patients with poor prognosis from those who benefited from chemotherapy. Therefore, there is an urgent need for a reliable molecular marker to identify high-risk g...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G16B20/00G16B40/00
CPCC12Q1/6886G16B20/00G16B40/00C12Q2600/158C12Q2600/178C12Q2600/118
Inventor 练磊熊志中李宪哲殷实谢明颢
Owner THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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