Method for extracting, separating and purifying aescin from semen aesculi

A escin, separation and purification technology, applied in the field of medicine, can solve the problems of increasing the difficulty of aescin purification, increasing the cost of aescin extraction, and complex components of the extract, so as to improve the effect of removing impurities and reduce production costs , low cost effect

Active Publication Date: 2021-10-22
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although aescin can be fully extracted from sunflower seeds, the components of the extract are too complex, requiring the use of ultra-high pressure extraction equipment, repeated pressure boosting and pressure relief, which increases the extraction cost of aescin and increases the cost of aescin. The Difficulty of Purifying Phyllosides

Method used

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  • Method for extracting, separating and purifying aescin from semen aesculi
  • Method for extracting, separating and purifying aescin from semen aesculi
  • Method for extracting, separating and purifying aescin from semen aesculi

Examples

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Embodiment 1

[0038] Weigh 200g of Salazi (Zhejiang Horse Chestnut) and crush it to 40-200 mesh, add it to the flash extraction-ultrasonic instrument, then add 2L of ethanol aqueous solution with a volume concentration of 50%, flash extraction (room temperature 25°C): normal rotation extraction : Extract at 5000r / min for 5min, 10000r / min for 3min, 15000r / min for 2min; reverse extraction: extract at 5000r / min for 5min, 10000r / min for 3min, 15000r / min for 2min; settle for 10min, remove the supernatant Liquid, get the first precipitate and the first clear liquid;

[0039] Then add 1.2L of 50% ethanol aqueous solution to the first precipitate, ultrasonically extract for 30min (38-44°C, 53KHz), settle for 10min, remove the supernatant to obtain the second precipitate and the second clear solution; then add 0.8L ethanol aqueous solution with a volume concentration of 50% to the second precipitate for ultrasonic extraction for 30min (41-45°C), settle for 10min, and remove the supernatant to obtain...

Embodiment 2

[0043] Weigh 0.5Kg of Sala seeds (Zhejiang Aesculus) and crush it to 40-200 mesh and add it to the flash extraction-ultrasonic instrument, then add 50% ethanol 5L, flash extraction (room temperature 25°C): forward rotation extraction: at 5000r / min extraction for 5 min, 10000r / min for 3min, 15000r / min for 2min; reverse extraction: extract at 5000r / min for 5min, 10000r / min for 3min, and 15000r / min for 2min; settle for 10min, remove the supernatant to obtain the - precipitate and first clear solution;

[0044] Then add 3 L of ethanol aqueous solution with a volume concentration of 50% to the first precipitate, ultrasonically extract for 30 min (38-43° C., 53 KHz), settle for 10 min, and remove the supernatant to obtain the second precipitate and the second clear liquid; Then add 50% ethanol aqueous solution 2L ultrasonic extraction 30min (40-43 ℃, 53KHz) to the second precipitate, let it settle down for 10min, remove the supernatant to obtain the third precipitate and the third ...

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Abstract

The invention discloses a method for extracting, separating and purifying aescin from semen aesculi, which comprises the following steps: carrying out flash extraction-ultrasonic extraction and filtration separation by using 50% low-concentration ethanol to obtain a high-polarity component extracting solution, repeatedly performing adsorbing and desorbing for three times by using D101 macroporous adsorption resin, performing eluting by using water and ethanol with different concentrations, collecting aescin fractions, putting the concentrated solution purified for the third time into a vessel, performing freeze-drying until white floccules are adhered to the vessel and yellow impurities such as saccharides are adhered to the bottom of the vessel, and collecting the white floccules to obtain the aescin with the purity of 96.5% or above. According to the method, the extraction and purification steps are simplified, and the production cost is saved; the method is simple to operate, easy to repeat and suitable for standard industrial production.

Description

technical field [0001] The invention relates to a method for extracting, isolating and purifying aescin from sunflower seeds, and belongs to the technical field of medicine. Background technique [0002] Aesculi is one of the traditional Chinese medicines recorded in "Chinese Pharmacopoeia". The traditional Chinese medicine Aesculi refers to Aesculus chinensis Bge., Aesculus chinensis var. Chestnut (A esculus wilsoniiRehd.) and other dry mature seeds, these three kinds of medicinal plants are special products of our country. Sala seed is warm in nature, sweet in taste, returns to the liver and stomach meridians, has the effect of regulating qi, relaxing the middle, neutralizing the stomach and relieving pain. It is mainly used to treat chest and abdomen distension and stomach pain. [0003] Aescin is an important active ingredient in the extract of Sala chinensis. Aescin has significant effects in anti-inflammation, anti-exudation, and detumescence. It can restore the norma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H15/256C07J63/00
CPCC07H1/08C07H15/256C07J63/008Y02P20/582
Inventor 张大帅史载锋陈文豪陈光英李晨
Owner HAINAN NORMAL UNIV
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