Nucleic acid synthesis reaction device and nucleic acid synthesis method

A synthetic reaction and nucleic acid technology, which is applied in bioreactor/fermenter combination, stress-stimulated microbial growth method, biochemical cleaning device, etc., can solve the problems of insufficient reaction effect and insufficient use of reaction reagents, etc., and achieve improvement Full utilization, sufficient response, and avoiding the effect of loss

Active Publication Date: 2022-03-08
北京擎科生物科技股份有限公司
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Problems solved by technology

[0004] Based on this, it is necessary to provide a new nucleic acid synthesis reaction device and nucleic acid synthesis method for the problems of insufficient reaction effect or insufficient use of reaction reagents in the traditional column synthesis method

Method used

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  • Nucleic acid synthesis reaction device and nucleic acid synthesis method
  • Nucleic acid synthesis reaction device and nucleic acid synthesis method
  • Nucleic acid synthesis reaction device and nucleic acid synthesis method

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preparation example Construction

[0035] Correspondingly, the present invention also provides a nucleic acid synthesis method, using the nucleic acid synthesis reaction device of any embodiment of the present invention, and comprising the following steps:

[0036] The magnetic nucleic acid synthesis carrier 2 and the nucleic acid synthesis reaction solution are loaded on the positive U-shaped part 12 of the reaction main tube 1 for reaction, and gas is passed into the positive U-shaped part 12 through the gas supply device 12 during the reaction process , so that the magnetic nucleic acid synthesis carrier 2 and the nucleic acid synthesis reaction solution are inflated and shaken;

[0037] After the reaction is over, the gas flow is stopped, so that the magnet 3 is attached to the outer surface of the positive U-shaped part 12, and then the waste liquid after reaction in the reaction main pipe 1 is discharged.

[0038]The nucleic acid synthesis reaction device of the present invention is a brand-new nucleic ac...

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Abstract

The invention discloses a nucleic acid synthesis reaction device, comprising: a reaction main pipe, the shape of the reaction main pipe is an "N" mirror image structure including a positive U-shaped part and an inverted U-shaped part structure, both ends have a liquid inlet and a liquid outlet, and the positive The U-shaped portion is closer to the liquid inlet, the height difference between the liquid outlet and the top of the inverted U-shaped portion, the height difference between the liquid inlet and the bottom of the positive U-shaped portion are greater than the height difference between the bottom of the positive U-shaped portion and the top of the inverted U-shaped portion; the magnet ; A gas supply device and a gas delivery pipe, one end of the gas delivery pipe communicates with the gas supply device, and the other end communicates with the reaction main pipe. The invention also discloses a nucleic acid synthesis method.

Description

technical field [0001] The invention relates to the technical field of nucleic acid synthesis, in particular to a nucleic acid synthesis reaction device and a nucleic acid synthesis method. Background technique [0002] Artificially synthesized DNA is a known important way to modify gene sequences, and it is widely used in many fields such as protein modification and life sciences, such as nucleic acid drugs, enzyme engineering, gene detection, gene therapy, etc. [0003] At present, the most common and widely used method in the market is the column synthesis method, which is mainly based on the controlled pore glass (Controlled Pore Glass, CPG) carrier for chemical synthesis, but the CPG carrier has limitations. Compared with the enzyme synthesis method with more efficient synthesis efficiency in frontier research, the CPG carrier pore size is too large, which will cause the loss of enzyme, and the pore size is too small or the column space is small, which will cause blocka...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42C12M1/04C12M1/00C12N15/10
CPCC12N15/1013
Inventor 马石金朱强
Owner 北京擎科生物科技股份有限公司
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