Culture method of coenzyme q10 production strain

Abstract

The invention relates to a culture method of a coenzyme q10 production strain. The culture method of the coenzyme q10 production strain comprises the steps of culture medium preparation, strain purification, mother bottle inoculation, second-stage bottle inoculation and third-stage fermentation bottle inoculation. According to the invention, by improving the formulas of a slant culture medium, a mother bottle culture medium, a seed culture medium and a fermentation culture medium, the growth of the strain can be accelerated, the slow period is short, the strain can be industrially and stably produced on a large scale, the coenzyme production capacity of the strain can be improved, the physiological state is stable, and the stable production capacity is maintained; and a seed expanding process is simplified, and a fermentation process is easier to arrange.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for cultivating coenzyme q10-producing strains. Background technique [0002] Coenzyme q10 (2,3-dimethoxy-5-methyl-6-decenylbenzoquinone, CoenzymeQ10, CoQ10), also known as ubiquinone, decenequinone, widely exists in animals, plants and microorganisms, is a biological An important hydrogen transporter in the cellular respiratory chain. Coenzyme Q10 is an important biochemical drug, which has been widely used in the treatment of various heart diseases, diabetes, cancer, acute and chronic hepatitis, Parkinson's disease and other diseases in recent years. In addition, it also has significant effects in treating scurvy, duodenal ulcer, necrotizing periodontitis, and promoting pancreatic function and secretion. At the same time, coenzyme q10 also has anti-aging effects, and has a wide range of markets in the fields of cosmetics and health products...

AI Technical Summary

Problems solved by technology

[0004] At present, the market demand for coenzyme q10 in China is increasing year by year, but when the culture medium used to cultivate coenzyme q10 production strains, the growth rate of the strains is slow, and the coenzyme q10 produced by the strains in the fermentation process The output is not high, and it is far from meeting the demand of the market.

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