Animal-derived component detection method based on high-throughput sequencing technology and application thereof

Abstract

The invention discloses an animal-derived component detection method based on a high-throughput sequencing technology and application thereof, and belongs to the technical field of biology. The method comprises the following steps of: (1) extracting DNA of a sample; (2) performing one-step PCR amplification; (3) performing MiSeq sequencing; and (4) performing SAFESeqr Suite data analysis. According to the animal-derived component detection method based on the high-throughput sequencing technology, the extracted DNA of the sample is directly subjected to high-throughput sequencing analysis, so that enough data volume is guaranteed; a universal primer is designed, so that meat products doped with a plurality of different species can be determined at the same time, the relative content of each species in the meat products can be determined, and quick identification and accurate determination of adulteration and doping of unknown animal-derived components are realized; and by adopting the method, components and components of 96 mixed samples can be analyzed at the same time, so that the method is high in sensitivity, simple to operate, low in cost and short in detection time.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for detecting animal-derived components based on high-throughput sequencing technology and its application. Background technique [0002] In recent years, my country's food industry has entered a golden age of rapid development. Poultry meat products and their products have enriched the dining tables of the masses. Meat food is the main channel for people to obtain high-quality protein. The quality of meat food is comparable to that of human beings. Health is closely related. However, in recent years, food adulteration has frequently occurred. Meat adulteration is a relatively common type of food adulteration. It is mainly because some illegal traders mix or replace meat products with similar forms, Non-identical meats that are less expensive. Take the "horse meat incident" as an example. In 2013, horse meat was detected in beef products in many large supermarke...

AI Technical Summary

Problems solved by technology

However, these techniques can only detect one or two indicators at a time, and cannot comprehensively analyze and detect various animal-derived ingredients involved in food. When encountering mixed samples of several different varieties, it is necessary to determine the The relative amount in the mixture needs to find the only known target sequence in the variety as a reference sequence, and it is very difficult to obtain accurate measurement results, and the existing detection methods have high requirements for operator skills and laboratory equipment configuration. The cost is high, and it is difficult to meet the detection requirements of high efficiency, fast, large batch, large scale, and simple operation, and cannot meet the urgent needs of market supervision, real-time control of raw material and finished product quality

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