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Multi-channel appliance for detecting color preference of juvenile zebrafish as well as using method and application of multi-channel appliance

A color preference and zebrafish technology, applied in the field of multi-channel equipment, can solve problems such as inaccurate results, zebrafish noise interference, time-consuming and labor-intensive problems, and achieve high throughput, avoid personnel interference, and reliable results

Active Publication Date: 2022-01-21
HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the prior art, the traditional four-color maze experiment is usually used to study the color preference of zebrafish, which has certain defects: 1) taking pictures at the end point of the experiment to determine the position where the zebrafish stays, which only represents the instantaneous result of the end point of exposure, There is chance; 2) Zebrafish are susceptible to noise interference caused by photography, resulting in inaccurate results; 3) The throughput of the cross maze experiment is small, time-consuming and laborious

Method used

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  • Multi-channel appliance for detecting color preference of juvenile zebrafish as well as using method and application of multi-channel appliance
  • Multi-channel appliance for detecting color preference of juvenile zebrafish as well as using method and application of multi-channel appliance
  • Multi-channel appliance for detecting color preference of juvenile zebrafish as well as using method and application of multi-channel appliance

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] like figure 1 and figure 2 Shown is a multi-channel apparatus for detecting color preference in larval zebrafish, including a mold body 3 and a monochrome module 2 . The upper surface of the mold main body 3 is provided with 12 rectangular transparent mold pools 1 . The monochrome module 2 includes 12 blue modules, 12 yellow modules, 12 red modules and 12 green modules. The monochrome module 2 is provided with a groove 2-1 matching the end of the transparent mold cell 1, and the end of the transparent mold cell 1 can be installed in the groove 2-1 of the monochrome module 2; all The grooves 2-1 on the monochrome modules 2 have the same shape and size. Both the mold main body 3 and the outer wall of the transparent mold pool 1 are cut from acrylic plates.

[0059] The length, width and thickness of the mold main body 3 are 125 mm, 85 mm and 5 mm, respectively. The inner length, inner width, inner height and wall thickness of the transparent mold cell 1 are 30mm, 10...

Embodiment 2

[0061] The difference between this embodiment and Embodiment 1 is that in this embodiment, the internal length, internal width, internal height and wall thickness of the transparent mold cell 1 are 27mm, 8mm, 8mm and 1.5mm respectively; The opening width, opening depth and height of 2-1 are 11 mm, 8 mm and 8 mm, respectively.

Embodiment 3

[0063] The only difference between this embodiment and Embodiment 1 is that in this embodiment, the internal length, internal width, internal height and wall thickness of the transparent mold cell 1 are 33mm, 12mm, 12mm and 2.5mm respectively; The opening width, opening depth and height of 2-1 are 17mm, 12mm and 12mm, respectively.

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Abstract

The invention relates to the technical field of zebra fish juvenile fish behavior testing, and discloses a multi-channel appliance for detecting color preference of juvenile zebrafish as well as a using method and application of the multi-channel appliance. The multi-channel appliance comprises at least one transparent mold small pool and at least two monochromatic modules, wherein all the monochromatic modules have at least two different colors; grooves matched with the end parts of the transparent mold small pools are formed in the monochromatic modules, and the end parts of the transparent mold small pools can be arranged in the grooves of the monochromatic modules; and the grooves in all the monochromatic modules are the same in shape and size. According to the multi-channel appliance for detecting color preference of the juvenile zebrafish as well as the using method and application of the multi-channel appliance, contingency and man-made interference can be reduced, and the reliability and detection flux of a detection result are improved; and meanwhile, by detecting the total movement distance of the juvenile zebrafish in different color areas, the preference strength of the juvenile zebrafish to a certain color can be quantitatively analyzed, and transverse comparison between different groups of juvenile zebrafish and longitudinal comparison of the juvenile zebrafish before and after certain specific treatment are realized.

Description

technical field [0001] The invention relates to the technical field of zebrafish behavior testing, in particular to a multi-channel device for detecting the color preference of juvenile zebrafish and its use method and application. Background technique [0002] Cognition is an intelligent processing process for the body to recognize and acquire knowledge, involving a series of random, psychological and social behaviors such as learning, memory, language, thinking, spirit, and emotion. Dementia is a disease in which brain function declines due to brain neuron lesions. Patients' memory, comprehension, language, learning, calculation, and judgment abilities will all be affected, and there will be some changes in mood, behavior, and sensation. At present, the common factors leading to cognitive impairment mainly include age, genetic factors, brain diseases, trauma and drug-induced factors. The treatment for cognitive impairment varies according to the pathogenic factors. In add...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K63/00A01K61/10A01K29/00
CPCA01K63/003A01K63/006A01K61/10A01K29/005Y02A40/81
Inventor 缪文钰夏波何玲玲张涛李春启
Owner HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
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