Method for disinfecting root surface of achyranthes bidentata and separating and culturing endophytic fungi of achyranthes bidentata

A technique of separating and cultivating Achyranthes bidentata, which is applied in the direction of separating microorganisms, fungi, disinfectants, etc., and can solve problems such as not seeing Achyranthes bidentata

Inactive Publication Date: 2022-01-21
XUCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no relevant research reports on the surface disinfection of Achyranthes bidentata roots and the isolation of endophytic fungi at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preferred embodiment of the present invention provides a method of disinfecting and isolating the surface of a null knee root, and the specific steps are as follows:

[0021] Choose a year of healthy black knees, the root was selected for 6 min, 4 wt% hypochlorite oscillate, and sterilized water was washed 3 times. The surrounding of the material after disinfection was discarded, and the remaining null's knee cated in the middle tissue of 1.5-2.5 mm inoculated into the PDA medium containing whipycin and streptomycin and containing the knee extract of the null knee extract. However, in which the content of hemithromin is 0.08 g / L, the content of streptomycin is 0.06 g / L, and the content of the null knee leaching is 90 mL / L.

Embodiment 2

[0023] Choose a year of healthy black knee, the root of the collected roots were sequentially extracted with 75% ethanol soaking 30s, 3 wt% hypochlorite oscillation washed for 7 min, and the sterile water was rinsed 4 times for disinfection treatment. The surrounding of the material after disinfection was discarded, and the remaining null's knee cated in the middle tissue of 1.5-2.5 mm inoculated into the PDA medium containing whipycin and streptomycin and containing the knee extract of the null knee extract. However, in which the content of penicillin is 0.07 g / L, the content of streptomycin is 0.07 g / L, and the content of the null knee leaching is 100 mL / L.

Embodiment 3

[0025] Choosing a year of healthy black knee and collected roots were sequentially extracted from 75% ethanol soaking 50s, 5 wt% hypochlorite oscillation washed 8 min, and sterile water rinsing 5 times for disinfection treatment. The surrounding of the material after disinfection was discarded, and the remaining null's knee cated in the middle tissue of 1.5-2.5 mm inoculated into the PDA medium containing whipycin and streptomycin and containing the knee extract of the null knee extract. However, in which the content of penicillin is 0.04 g / L, the content of streptomycin is 0.05 g / L, and the adverse liquid content of the null knee is 120 ml / L.

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PUM

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Abstract

The invention discloses a method for disinfecting the root surface of achyranthes bidentata and separating and culturing endophytic fungi of the achyranthes bidentata, and belongs to the technical field of fungus separating and culturing. The method comprises the following steps of selecting one-year-old healthy achyranthes bidentata roots, and sequentially soaking the achyranthes bidentata roots with 75% ethanol for 30-50s, performing oscillation washing with 3-5wt% sodium hypochlorite for 6-8min, and rinsing with sterile water for 3-5 times for disinfection treatment; cutting and discarding surrounding tissues of the disinfected achyranthes bidentata root material, and inoculating the residual middle tissues to a PDA culture medium containing penicillin and streptomycin and a PDA culture medium containing achyranthes bidentata extract liquid for culturing. According to the method disclosed by the invention, the surface of the achyranthes bidentata tissue is disinfected, and endophytic fungi are protected from being damaged while the disinfection effect is ensured.

Description

Technical field [0001] The present invention belongs to the field of fungi separation culture, and in particular to a method of disinfecting the surface of a null's knee root and separating its endogenous fungi. Background technique [0002] Achyranthes Bidentata Blume.) Achyranthes is a deep rooted grass plant, which is growing in a hillside of 200-1750 meters above sea level, which is mainly produced in the Jiaozuo area of ​​Henan Province, Fuyang, Meng State, Bo love, Xiuwu, Wenxian (the ancient Huaiqingfu). The unique geographic landforms and climate characteristics of this area ensure the biological independence of species. As a result, Huaiqingfu kines may have potential endometric species resources with other regions, other species. [0003] Separating the endogenic fungi from the plant tissue is the basis for studying its function and diversity, and a large number of studies on endogenous fungi involve separating endogenic fungi from host plants. During the process of sep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02A01N59/00A01N31/02A01P1/00
CPCC12N1/14C12N1/02A01N59/00A01N31/02
Inventor 孙思胜李光辉郭孝辉
Owner XUCHANG UNIV
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