Application of propanamide in preparation of nematicidal preparations
A technology of insecticide and propionamide, which is applied in the application field of propionamide in the preparation of nematicide preparations, can solve the problems of non-compliance, low efficiency, unfriendly chemicals and the environment, and achieve good sustainability and improved insecticidal ability  Effect
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Embodiment 1
[0021] The application of propionamide in the preparation of the preparation for killing root-knot nematode incognita, the application process is as follows:
[0022] Pick nematode egg masses from the roots of tomatoes infected with M. incognita, place the egg masses in a petri dish filled with 15 mL of sterile water and incubate in the dark at 20°C to observe the hatching of M. incognita. Nematodes were collected by centrifugation and the nematode concentration was calculated.
[0023] will be purchased from The company's propionamide was formulated into a 1mg / mL aqueous solution, added to a 96-well plate, and the final concentration of propionamide was 200, 100, 50, 25, 12.5, 6.25 μg / mL by adding sterile water. 30-40 nematodes, the total bioassay system was 100 μL, sterile water was used as negative control, and each group was repeated 3 times. Place them in a 20°C incubator for 48 hours in the dark and culture them under a microscope to count the mortality of the nematod...
Embodiment 2
[0028] The application of propionamide in preparation killing root-knot nematode javanica, application process is as follows:
[0029] Pick nematode egg masses from tomato roots infected with root-knot nematode javanica, place the egg masses in a petri dish filled with 15 mL of sterile water and incubate in the dark at 20°C, and observe the hatching of root-knot nematodes. Nematodes were collected by centrifugation and the nematode concentration was calculated.
[0030] Add 1 mg / mL propionamide aqueous solution into a 96-well plate, so that the final concentration of propionamide is 400, 200, 100, 50, 25, 12.5 μg / mL, add 30-40 nematodes to each well, and the total bioassay system For 100 μL, sterile water was used as negative control, and each group was repeated 3 times. Placed in a 20°C incubator for 48 hours in the dark and cultured under the microscope to count the nematode mortality. The activity of propionamide against root-knot nematode javanica is shown in Table 2, LC ...
Embodiment 3
[0035] The application of propionamide in the preparation of preparations for killing soybean cyst nematodes, the application process is as follows:
[0036] Pick nematode cysts from soybean roots infected with soybean cyst nematode (Heterodera glycines), rub the cysts to release eggs, place them in a petri dish filled with 15 mL of sterile water and incubate in the dark at 20°C, and observe the hatching of root-knot nematodes . Nematodes were collected by centrifugation and the nematode concentration was calculated.
[0037] Add 1 mg / mL propionamide aqueous solution into a 96-well plate, so that the final concentration of propionamide is 400, 200, 100, 50, 25, 12.5 μg / mL, add 30-40 nematodes to each well, and the total bioassay system For 100 μL, sterile water was used as negative control, and each group was repeated 3 times. Placed in an incubator at 20°C for 48 hours in the dark and cultured under a microscope to count nematode mortality, the activity of propionamide agai...
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