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Application of propanamide in preparation of nematicidal preparations

A technology of insecticide and propionamide, which is applied in the application field of propionamide in the preparation of nematicide preparations, can solve the problems of non-compliance, low efficiency, unfriendly chemicals and the environment, and achieve good sustainability and improved insecticidal ability Effect

Active Publication Date: 2022-02-15
HUBEI BIOPESTICIDE ENG RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the efficiency of agricultural control and physical control is low, which does not meet the needs of modern agricultural development; chemical control is the main control method at present, but some chemicals are not friendly to the environment, and the current resistance problem of nematodes has become increasingly serious; biological control It is the use of natural enemies of nematodes or biological natural products with nematicidal activity to control plant nematodes

Method used

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  • Application of propanamide in preparation of nematicidal preparations
  • Application of propanamide in preparation of nematicidal preparations
  • Application of propanamide in preparation of nematicidal preparations

Examples

Experimental program
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Effect test

Embodiment 1

[0021] The application of propionamide in the preparation of the preparation for killing root-knot nematode incognita, the application process is as follows:

[0022] Pick nematode egg masses from the roots of tomatoes infected with M. incognita, place the egg masses in a petri dish filled with 15 mL of sterile water and incubate in the dark at 20°C to observe the hatching of M. incognita. Nematodes were collected by centrifugation and the nematode concentration was calculated.

[0023] will be purchased from The company's propionamide was formulated into a 1mg / mL aqueous solution, added to a 96-well plate, and the final concentration of propionamide was 200, 100, 50, 25, 12.5, 6.25 μg / mL by adding sterile water. 30-40 nematodes, the total bioassay system was 100 μL, sterile water was used as negative control, and each group was repeated 3 times. Place them in a 20°C incubator for 48 hours in the dark and culture them under a microscope to count the mortality of the nematod...

Embodiment 2

[0028] The application of propionamide in preparation killing root-knot nematode javanica, application process is as follows:

[0029] Pick nematode egg masses from tomato roots infected with root-knot nematode javanica, place the egg masses in a petri dish filled with 15 mL of sterile water and incubate in the dark at 20°C, and observe the hatching of root-knot nematodes. Nematodes were collected by centrifugation and the nematode concentration was calculated.

[0030] Add 1 mg / mL propionamide aqueous solution into a 96-well plate, so that the final concentration of propionamide is 400, 200, 100, 50, 25, 12.5 μg / mL, add 30-40 nematodes to each well, and the total bioassay system For 100 μL, sterile water was used as negative control, and each group was repeated 3 times. Placed in a 20°C incubator for 48 hours in the dark and cultured under the microscope to count the nematode mortality. The activity of propionamide against root-knot nematode javanica is shown in Table 2, LC ...

Embodiment 3

[0035] The application of propionamide in the preparation of preparations for killing soybean cyst nematodes, the application process is as follows:

[0036] Pick nematode cysts from soybean roots infected with soybean cyst nematode (Heterodera glycines), rub the cysts to release eggs, place them in a petri dish filled with 15 mL of sterile water and incubate in the dark at 20°C, and observe the hatching of root-knot nematodes . Nematodes were collected by centrifugation and the nematode concentration was calculated.

[0037] Add 1 mg / mL propionamide aqueous solution into a 96-well plate, so that the final concentration of propionamide is 400, 200, 100, 50, 25, 12.5 μg / mL, add 30-40 nematodes to each well, and the total bioassay system For 100 μL, sterile water was used as negative control, and each group was repeated 3 times. Placed in an incubator at 20°C for 48 hours in the dark and cultured under a microscope to count nematode mortality, the activity of propionamide agai...

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Abstract

The invention relates to the technical field of agricultural microbiology, and in particular, relates to an application of propionamide in preparation of nematicidal preparations, andthrough determination of the fatality rates of meloidogyne incognita, meloidogyne javanica and soybean cyst nematodes, it is found that the propionamide has good insecticidal activity on nematodes, the LC50 values of the propionamide on M.incognita, M.javanica and heterodera glycines are 22.87 [mu]g / mL, 107.02 [mu]g / mL and 46.09 [mu]g / mL respectively, after the propionamide is compounded with betaine, the lowest LC50 value of the compound preparation on the M.incognita is 13.92 [mu]g / mL, and after the compound is compounded with methylmalonic acid, the lowest LC50 value of the compound preparation on the M.incognita is 14.48 [mu]g / mL. The preparations provide a new resource for research and development of a novel plant nematode killing preparation.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbiology, and in particular relates to the application of propionamide in the preparation of nematicide preparations. Background technique [0002] More than 5,000 species of nematodes capable of parasitic plants have been found in more than 200 genera. Among them, root-knot nematodes (Meloidogyne spp.) and cyst nematodes (Heterodera spp. and Globodera spp.) are the most serious. [0003] Root-knot nematodes can parasitize almost all vascular plants, including southern root-knot nematode (M.incognita), northern root-knot nematode (M.hapla), Javan root-knot nematode (M.javanica) and peanut root-knot nematode (M.arenaria ) is the most serious harm, and the harm of root-knot nematode (M.enterolobii) in my country has become increasingly serious in recent years. The female of root-knot nematode lays eggs in an egg mass composed of a protective colloidal matrix. After embryonic development, t...

Claims

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Application Information

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IPC IPC(8): A01N37/18A01N37/44A01N37/04A01P5/00
CPCA01N37/18A01N37/44A01N37/04Y02A50/30
Inventor 陈凌刘晓艳刘南茜王梦思闵勇邱一敏朱镭周荣华田雨曦饶犇廖先清陈伟王月莹
Owner HUBEI BIOPESTICIDE ENG RES CENT
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