Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for enhancing efficacy of stem cells by using ethionamide

A technology of ethionamide and stem cells, which is applied in the field of medium compositions for enhancing stem cell efficacy, can solve problems such as insufficient development, prevent or treat inflammatory diseases or degenerative brain diseases, and improve anti-inflammatory effects. , anti-inflammatory effect improvement effect

Pending Publication Date: 2022-03-01
SAMSUNG LIFE PUBLIC WELFARE FOUND
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, since only the above-mentioned effects of mesenchymal stem cells are known, and the development of mesenchymal stem cells optimized to have more improved therapeutic effects has not been fully developed, there is an urgent need to develop Stem cell therapy optimized for efficacy in brain diseases

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for enhancing efficacy of stem cells by using ethionamide
  • Method for enhancing efficacy of stem cells by using ethionamide
  • Method for enhancing efficacy of stem cells by using ethionamide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1. Preparation of human umbilical with metamorite stem cells

[0083] After obtaining the umbilical cord after approved by IRB (IRB # 2015-09-023-003) according to Samsung Medical Center, the human umbilical-cord serum is separated by the following method.

[0084] First, a 3 to 4 cm of umbilical cord tissue was combined, and the collagen enzyme solution (GIBCO, USA) was treated with an extracellular matrix, and then 0.25% trypsin (GIBCO, USA) was digested at 37 ° C for 30 minutes. . Subsequently, the cells were obtained by adding a fetal bovine serum (FBS) (Biowest, USA) to the resulting product, then centrifuged at 1000 × g for 10 minutes, then use 10% FBS and 50μg / ml to celebrate MEM medium (Minimum Essential Media) (GIBCO, USA) (GIBCO, USA) (GIBCO, USA) at 37 ° C, 5% CO 2 The environment is cultured, thereby passing between 5 or 6 mesenchymal stem cells being used in experiments.

Embodiment 2

[0085] Example 2. Preparation of human umbilical-tape mesenchymal stem cells passing through vs.

[0086] 6 × 10 3 Cell / cm 2 The human umbilical binding mesenchymal stem cells prepared according to Example 1 were treated in a cell culture vessel while 50 μm, 100 μm or 150 μm concentrations were treated with ethylene isoparamia, and then cultured for 72 hours.

Embodiment 3

[0087] Example 3. Verification of stem cell anti-inflammation effect

[0088] In order to demonstrate whether the anti-inflammatory effect of stem cells is improved when treating mesenchymal stem cells with ethylene isoparamia, and performs the following experiments. Specifically, the inflammatory model is to induce inflammatory model by treating microglia Bv2 cells with LPS (lipopolysaccharide, LipopolysAccharide), and is also mixed with EtOAc. Coute, subsequently measured nitric oxide; NO), NO-related factor and the expression level of the active oxygen.

[0089] As a result, if Figure 1A As shown in the case of representative NO-related inflammatory factors, INOS (induced nitric oxide synthase, inducible nitric oxide synthase), metallic stem cells with control or with ethylene Compared with the co-cultured group, mRNA expression was significantly reduced in groups of methatetransfeitrous stem cells with vs. In addition, if Figure 1B and 1c As shown, according to the results of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a culture medium composition for enhancing the efficacy of stem cells, the culture medium composition comprising ethionamide; a method for enhancing the efficacy of stem cells and a method for producing stem cells having enhanced efficacy, comprising a step of culturing stem cells in the culture medium composition; stem cells produced by the method and uses thereof. According to the present invention, a simple process of treating mesenchymal stem cells with ethionamide can effectively enhance the anti-inflammatory action of the mesenchymal stem cells and the expression level of paracrine factors, and the stem cells obtained by the method can be used for preventing or treating inflammatory diseases or degenerative brain diseases.

Description

Technical field [0001] The present invention relates to a medium composition for enhancing stem cell energy, which comprises ethioxisethionamide; a method of enhancing stem cells, which comprises culturing stem cells in a medium composition; a method of preparing stem cells having enhanced efficacy Stem cells and their uses prepared by the method. Background technique [0002] Inflamimation is one of the biological reactions such as biological tissue such as pathogens and damaged cells, and protective responses relating to immunocytes, vascular and molecular organizers. However, abnormal inflammation is associated with various human diseases and leads to various diseases, such as allergies, specialty, arthritis, heart disease, brain disease, circulating disorders, and cancer. The occurrence of various inflammatory diseases is associated with activation of macrophages, which leads to excessive production of inflammatory factors, and representative inflammatory factors include inte...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/0735C12N5/0775A61K35/28A61K35/545A61P25/00A61P29/00
CPCC12N5/0606C12N5/0668A61K35/545A61K35/28A61P25/00A61P29/00C12N2501/999A61P25/28A61K35/51C12N5/0665
Inventor 罗德烈蒋锺旭孙孝真
Owner SAMSUNG LIFE PUBLIC WELFARE FOUND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com