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Kit, composite system and application thereof in obtaining individual age

A kit and system technology, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of high cost of kits, inapplicability to Chinese population, narrow gender and age of application, and achieve cost Low, good large-scale application prospects, similar accuracy effects

Pending Publication Date: 2022-03-11
北京中科华生基因医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention provides a kit, a composite system and its application in obtaining individual age in order to solve the problems that the existing kit for obtaining individual age is too high in cost, unsuitable for the Chinese population, narrow in gender and age, etc.

Method used

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  • Kit, composite system and application thereof in obtaining individual age
  • Kit, composite system and application thereof in obtaining individual age
  • Kit, composite system and application thereof in obtaining individual age

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Embodiment 1 A kind of test kit

[0117] Contains 20 amplification primers for amplifying 10 CpG sites in the genomic DNA of the sample, and its polynucleotide sequence is the sequence shown in SEQ ID No.1-SEQ ID No.20;

[0118] It contains 10 extended primers for obtaining the methylation rate of 10 CpG sites in the genomic DNA, and its polynucleotide sequence is the sequence shown in SEQ ID No.21-SEQ ID No.30.

[0119] The 10 CpG sites in the genomic DNA of the above samples include those corresponding to Illumina™ probe IDs cg16867657, cg08097417, cg13552692, cg10501210, cg06639320, cg26079664, cg07850154, cg18537454, cg01949323 and cG08 sites.

Embodiment 2

[0120] Example 2 Using the kit and composite system provided by the present invention to obtain the individual age of the Chinese population

[0121] 1. Sample selection

[0122] The blood samples of 14 unrelated individuals of different ages, different regions, and mixed sexes in the Chinese population were collected. The age range was between 24 and 64 years old. The specific information of the samples is shown in Table 13 below.

[0123] Table 13

[0124] Sample classification quantity percentage average value standard deviation maximum value minimum value female 8 57.14％ \ \ \ \ male 6 42.86％ \ \ \ \ age \ \ 33.07 10.79 \ \ age \ \ \ \ 24 64

[0125] 2. Genomic DNA was extracted from the above 14 blood samples.

[0126] 3. Obtain the methylation values ​​of the above 10 CpG sites in the genomic DNA of 14 blood samples by using the kit in Example 1.

[0127] 4. Bring the methylation values ​​of the 10 ...

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Abstract

The invention relates to the technical field of biology, and provides a kit, a compound system and application of the compound system in obtaining individual ages. The kit comprises an amplification primer used for amplifying at least one CpG site in 10 specific CpG sites in sample genome DNA and / or an extension primer used for obtaining the methylation rate of at least one CpG site in the 10 specific CpG sites, and the composite system comprises a genome DNA extraction system, an amplification detection system and a data acquisition system. The application is the application of the kit and the composite system in obtaining the age of an individual. The kit provided by the invention has the advantages of low cost, high speed and high accuracy for obtaining the methylation rate of 10 specific CpG loci in sample genome DNA, can be used for quickly and accurately deducing the age of an individual, is particularly suitable for being applied to the field of criminal investigation identification, and can quickly provide clues for case detection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit, a composite system and their application in obtaining the age of an individual. Background technique [0002] Human aging is associated with changes in DNA methylation at specific sites in the genome. These epigenetic modifications can be used to track donor age for forensic analysis or to assess biological age. Related studies have shown that the methylation level of specific CpG sites in the human genome will change with age. For some CpG sites, the degree of methylation is almost linearly correlated with age, and these CpG sites can be used as biomarkers for predicting age. Blood is a relatively common biological trace in crime scenes, and it is also the most widely used biological sample for age inference research based on DNA methylation. Predicting age by measuring the methylation level of specific CpG sites in blood has important application value in forensic science...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/154
Inventor 温冰涛史延凯曾庆良
Owner 北京中科华生基因医学科技有限公司
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