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Pseudomarine bacteria strain with obvious malathion degradation effect and application thereof

A technology of malathion and sea-source bacteria, applied in the field of biology

Pending Publication Date: 2022-03-15
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is how to degrade malathion in seawater, and / or how to control marine pollution

Method used

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  • Pseudomarine bacteria strain with obvious malathion degradation effect and application thereof
  • Pseudomarine bacteria strain with obvious malathion degradation effect and application thereof
  • Pseudomarine bacteria strain with obvious malathion degradation effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1. Screening and enrichment of sea-source bacteria malathion-degrading bacteria

[0077] 1. Isolation of Pseudidiomarina sp. R2059-CB1-ML CGMCC No.23970

[0078] Pseudidiomarina sp. R2059-CB1-ML CGMCC No.23970 was isolated from deep-sea paleothermal fluid samples in the South China Sea. The deep-sea paleohydrothermal samples in the South China Sea were sampled in May 2018 at N15.034363 and E116.522965 in the South China Sea. They were sediment samples at a water depth of 988m.

[0079] The 2216E solid medium plate was used for streak screening and purification at 30°C. The purified strains were respectively inserted into Erlenmeyer flasks equipped with 2216E liquid medium, and vibrated at 37° C. and 200 rpm for 10 h to obtain a fermentation broth cultivated in 2216E liquid medium for future use.

[0080] After R2059-CB1-ML was streak cultured on 2216E solid medium for 24 hours, the strain was round, light yellow and transparent, with a smooth and raised surfac...

Embodiment 2

[0084] Example 2, Degradation of Malathion by Pseudomonas spp. R2059-CB1-ML

[0085] The fermented liquid cultured in the 2216E liquid culture medium of Pseudomonas spp. R2059-CB1-ML strain preserved in embodiment 1 detects OD in ultraviolet spectrophotometer 600nm , collect OD 600nm =4 (with 2216E liquid medium as blank control) the fermentation broth was centrifuged at room temperature and 3500rpm for 8min, the supernatant was discarded, resuspended with an equal volume of inorganic salt liquid medium, washed 1-2 times, and the resuspended of fermentation broth. Two treatments were set up, the experimental group and the control group, as follows:

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Abstract

The invention discloses a pseudomarine bacterium strain with a remarkable malathion degradation effect and application of the pseudomarine bacterium strain. The malathion degrading bacterium disclosed by the invention is pseudomarine bacterium (Pseudomarine sp.) derived from deep sea hydrothermal liquid, the strain number of the pseudomarine bacterium is R2059-CB1-ML, and the registration number of the pseudomarine bacterium in the China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center) No. 23970. The strain can degrade malathion, can treat seawater pollution, and can be used for marine environment improvement.

Description

technical field [0001] The invention relates to a strain of the genus Pseudomonas genus which has a significant degrading effect on malathion in the field of biotechnology and its application. Background technique [0002] Organophosphorus compounds are synthetic highly toxic substances containing phosphorus, which are widely used in agricultural, industrial and national defense fields such as pesticides, flame retardants, plasticizers, and chemical weapons. Organophosphorus pesticides (organophosphorus pesticides, OPs) are widely used in agricultural production due to their high efficiency, broad spectrum, low residue and low cost, and malathion is one of them. [0003] Malathion is a highly efficient and highly toxic organophosphorus pesticide, widely used in the control of crop pests. Its accumulation in the environment and its toxicity are the greatest threats to the environment, while being lethal to beneficial insects, snails, microcrustaceans, fish, birds, amphibians...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A62D3/02C12R1/01
CPCC12N1/20C02F3/34A62D3/02C02F2101/30C02F2103/08A62D2101/26
Inventor 董志扬马羚王丽戴欣
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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