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Steroid compounds in ajuga ciliata bunge as well as extraction method and application thereof

A compound and separation method technology, applied in the field of medicine, can solve the problems of dependence on imports and high treatment costs, and achieve the effect of easy separation and strong inhibitory activity

Active Publication Date: 2022-03-22
XIAMEN INST OF RARE EARTH MATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the new anti-cancer drugs for clinical application basically rely on imports, and the cost of treatment is high

Method used

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  • Steroid compounds in ajuga ciliata bunge as well as extraction method and application thereof
  • Steroid compounds in ajuga ciliata bunge as well as extraction method and application thereof
  • Steroid compounds in ajuga ciliata bunge as well as extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1: Extraction, separation and structural identification of steroidal compounds A, B, C, D, E and F

[0070] The extraction and separation of compounds A, B and C is carried out according to the following process:

[0071] (a) Grind 20 kg of dried Herba sinensis (collected from Pingtan Island, Fujian Province in 2018), put them into two 20L plastic buckets, add 10L of 70% ethanol / water solution to soak at room temperature for 1 month, filter and concentrate That is the extract. The above-mentioned soaking, filtering, and concentrated extraction were repeated three times for the two barrels of medicinal materials, and the obtained extracts were combined.

[0072] (b) Disperse 1 kg of the extract obtained in step a in 1000 mL of water, and extract 5 times with petroleum ether, ethyl acetate and chloroform successively, each time the amount of petroleum ether, ethyl acetate and chloroform is 500 milliliters. Concentrate the extracts of different polarities respect...

Embodiment 2

[0084] Embodiment 2: in vitro antitumor activity test

[0085] Compound A was optimized for in vitro anti-tumor activity testing, mainly studying its in vitro inhibitory activity on lung cancer cell line A549, breast cancer cell line MCF-7, gastric cancer cell line (preserved and provided by Ningxia Medical University) and cervical cancer cell line . The specific testing process is illustrated by taking the testing process of the breast cancer MCF-7 cell line as an example: the specific process is as follows:

[0086] 1. Test sample concentration preparation

[0087] Weigh 3.5 mg of Compound A, add it to a 5 mL plastic centrifuge tube, and dilute to 2 mL with DMSO. The resulting concentration was an initial concentration of 1.75 mg / mL. Then the initial concentration was diluted with DMSO to obtain 6 different concentration gradients of 1.75mg / mL, 0.875mg / mL, 0.4375mg / mL, 0.21875mg / mL, 0.109375mg / mL and 0.0546875mg / mL in sequence. Store in refrigerator at 4°C until use.

...

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Abstract

The invention discloses a compound shown in a formula (I), and further discloses six compounds separated from ajuga ciliata and a separation method of the six compounds. The compound shown in the formula (I) has very strong inhibitory activity on a lung adenocarcinoma cell strain (A549), a gastric cancer cell strain, a breast cancer cell strain (MCF-7) and a cervical cancer cell strain (Hela), and can be used for candidate drugs or lead drugs for treating tumor / cancer diseases.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a novel steroid compound isolated from Herba chinensis, an extraction method thereof, and an application in treating tumor / cancer. Background technique [0002] Cancer has become the leading cause of death worldwide. Cancer can occur in various organs and tissues at any age. The World Health Organization's International Agency for Research on Cancer (IARC) released the latest global cancer statistics in 2020. The data shows that there will be 19.29 million new cancer cases worldwide in 2020, including 10.06 million males and 9.23 million females, and 9.96 million global cancer deaths in 2020, including 5.53 million males and 4.43 million females. In 2020, there will be 4.57 million new cancer cases in my country, including 2.48 million men and 2.09 million women. In 2020, there will be 3 million cancer deaths in my country, including 1.82 million men and 1.18 millio...

Claims

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Application Information

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IPC IPC(8): C07J17/00C07J9/00A61P35/00
CPCC07J17/00C07J9/00A61P35/00
Inventor 雍建平奥拉特德·奥拉戈克·扎克斯卢灿忠
Owner XIAMEN INST OF RARE EARTH MATERIALS
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