Naringenin chiral resolution method and absolute configuration determination method

Abstract

The invention belongs to the technical field of chiral resolution, and discloses a naringenin chiral resolution method and an absolute configuration determination method, and the method comprises the following steps: carrying out chiral resolution on naringenin by adopting a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS); conditions of the high performance liquid chromatography-tandem mass spectrometry method comprise: liquid phase conditions: adopting a bonded chiral chromatographic column, taking methanol-formic acid water as a mobile phase, and carrying out isocratic elution; mass spectrum conditions are as follows: an ESI ion source is adopted, and mass spectrum detection of chiral naringenin is carried out in a multi-reaction monitoring negative ion scanning mode. According to the method, a green and efficient ultrasonic synergistic technology is adopted, naringenin in citrus peels, pulp and whole fruits is extracted in a classified manner, the pretreatment conditions of citrus samples including the peels, the pulp and the whole fruits are better optimized, and the loss of target functional components is reduced. According to the present invention, the naringenin chiral resolution can be achieved, and the absolute configurations of the R-naringenin and the S-naringenin are determined;

Description

technical field [0001] The invention belongs to the technical field of chiral resolution, and in particular relates to a method for chiral resolution of naringenin and a method for determining the absolute configuration. Background technique [0002] At present, naringenin (Naringenin, NAR), belonging to dihydroflavonoids, is the aglycon of naringin, and mainly exists in citrus plants of Rutaceae such as grapefruit, orange, bergamot and other fruits. Naringenin is white needle crystal at room temperature, soluble in ethanol, ether and benzene, almost insoluble in water. Naringenin has antibacterial, anti-inflammatory, scavenging free radicals, anti-oxidation, cough and expectorant, lowering blood fat, anti-cancer and anti-tumor, antispasmodic and choleretic, prevention and treatment of liver disease, inhibition of platelet aggregation, anti-atherosclerosis, etc. This kind of pharmacological activity can be widely used in medicine, food and other fields. [0003] Naringenin...

AI Technical Summary

Problems solved by technology

[0005] Through the above analysis, the existing problems and defects of the prior art are: the prior art cannot realize the rapid chiral resolution of naringenin, and the absolute configuration of R and S of naringenin has not been determined at the same time

Therefore, it is impossible to carry out the extraction and preparation of chiral naringenin monomer and the biological efficacy analysis of the monomer

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